损伤性氧化反应在腹主动脉瘤形成中的作用  

Role of oxidative vascular injury in the pathogenesis of abdominal aortic aneurysms

作　　者：张健[1] 赵志青[2] 张平[1] 辛世杰[1] 张强[1] 段志泉[1] 

机构地区：[1]中国医科大学第一临床学院血管外科,沈阳110001 [2]上海第二军医大学长海医院血管外科

出　　处：《中华普通外科杂志》2006年第2期111-113,共3页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目(30471706)

摘　　要：目的探讨诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)在人腹主动脉瘤(abdominal aortic aneurysm,AAA)组织中的表达情况,了解一氧化氮(nitric oxide,NO)在人AAA形成中的作用。方法 22例AAA患者及10例正常人腹主动脉组织标本。采用原位杂交及免疫荧光染色的方法探查iNOS mRNA及蛋白的表达。采用原位杂交联合免疫荧光染色的方法探查阳性细胞的性质。采用免疫荧光染色检测过氧化亚硝酸盐。结果原位杂交和免疫荧光发现iNOS在22例 AAA组织中外膜均有表达。联合免疫荧光染色证实阳性细胞分别为:T、B淋巴细胞、巨噬细胞和平滑肌细胞(smooth muscle cell,SMC)。22例AAA均出现硝基酪氨酸的表达,阳性细胞为巨噬细胞和 SMC。10例正常腹主动脉组织均没发现有iNOS和硝基酪氨酸的表达。结论 iNOS的表达与人 AAA组织的退行性变关系密切。iNOS促进过氧化亚硝酸盐的生成,导致组织和细胞的氧化损伤。Objective To evaluate expression of inducible nitric oxide synthase （iNOS） in human abdominal aortic aneurysm （AAA） and to identify its initiation in the oxidative vascular injury. Methods This study included 22 AAA patients and 10 cadaveric normal abdominal aorta. In situ hybridization and immunofluorenscent staining were used to localize iNOS messenger RNA （mRNA） and protein. Double staining with a combination of in situ hybridization and immunofluorenscent staining was used to simultaneously demonstrate iNOS mRNA expression and its cellular localization. The presence of end-product of oxidative injury induced by iNOS was indirectly assessed with immunofluorenscent staining by anti-nitrotyrosine antibody, its cellular localization were assessed by double immunofluorenscent staining. Results In situ hybridization and immunohistochemistry confirmed the presence of iNOS in media and adventitia of AAA in all 22 patients. Specific cell markers identified iNOS mRNA-positive cells were T and B lymphocytes, macrophages, and smooth muscle cells. Positive immunostaining for nitrotyrosine was present in macrophages and smooth muscle cells. Normal abdominal aorta demonstrated virtually no iNOS or nitrotyrosine expression. Conclusion Stimulated expression of iNOS is associated with degeneration of AAA in human beings leading to oxidative tissue and cellular injury in AAA.

关 键 词：主动脉瘤 腹 一氧化氮 

分 类 号：R654.3[医药卫生—外科学]