紫红链霉菌对钉螺酶组织化学的影响  

Influence of Streptomyces violaceoruber on the enzyme-histochemistry in Oncomelania hupensis

作　　者：谭苹[1] 杨建明[2] 肖瑞芬[2] 张艳[3] 

机构地区：[1]武汉科技大学医学院寄生虫学教研室,武汉430080 [2]湖北大学生命科学学院,武汉430062 [3]华中科技大学同济医学院解剖学教研室,武汉430022

出　　处：《动物学报》2006年第1期109-114,共6页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金资助项目(No.30170179);湖北省血吸虫病专家咨询委员会资助课题~~

摘　　要：为研究紫红链霉菌灭螺作用的机理,将钉螺分别浸泡于紫红链霉菌培养液(含菌4×106/ml)及去氯水、培养基中36h后,用酶组织化学方法显示各组钉螺肝脏、中枢神经节、头足部及鳃的Mg2+激活的三磷酸腺苷酶(Mg2+ATPase)、胆碱脂酶(ChE)、一氧化氮合酶(NOS)、乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)并观察其变化。结果显示:菌液浸泡组钉螺的Mg2+ATPase活性在肝脏、中枢神经节、头足部及鳃部均明显减弱或完全失活,LDH在中枢神经节、头足部也有一定程度减弱,ChE、NOS、SDH在肝脏、中枢神经节、头足部及鳃部与去氯水组无明显差异;培养基组与去氯水组钉螺相应部位的Mg2+ATPase、ChE、NOS、LDH、SDH活性一致。结果提示:紫红链霉菌的灭螺作用机理主要在于破坏钉螺体内Mg2+ATPase和LDH活性,使ATP生成和利用障碍。This study examined the mechanisms of molluscicide from Streptomyces violaceoruber. After snails were immersed in the liquid with Streptomyces violaceoruber or the de-chlorinous water or the culture medium for 36 hours, the Mg^2＋-ATPase, ChE, NOS, LDH and SDH in the liver, centric ganglions, head-foot muscles and ctenidia of Oncomelania hupensis were characterized by enzyme-histoehemistry technology and changes of them were observed closely. Results show that the activity of Mg^2＋-ATPase in the liver, centric ganglions, head-foot muscles and ctenidia decreased noticably or disappeared after snails were immersed in the liquid with Streptomyces violaceoruber for 36 hours. The activity of LDH in centric ganglions, head-foot muscles of the snail decreased in some degree. There isn＇t much difference between the de-chlorinous water group and the liquid with Streptomyces violaceoruber group in the activities of NOS, ChE and SDH. The activities of Mg^2＋-ATPase, ChE, NOS, LDH and SDH at counterparts of the snail＇s respectively grown in de-chlorinated water and in the culture medium were identical. The results indicate the mechanism of molluscicide coming from Streptomyces violaceoruber is that the Mg^2＋ -ATPase and LDH are destroyed, and formation and release of ATP in the liver, centric ganglions, head-foot muscles and ctenidia are stopped. Cessation of vital functions casues deathof snails [Acta Zoologica Sinica 52 （1）： 109- 114, 2006].

关 键 词：钉螺 紫红链霉菌 胆碱脂酶乳酸脱氢酶 三磷酸腺苷酶 一氧化氮合酶 琥珀酸脱氢酶 组织化学 

分 类 号：R184.38[医药卫生—流行病学] Q954.62[医药卫生—公共卫生与预防医学]