纳米细菌的免疫电镜检测  

Detection of nanobacteria by immune electronic microscopy

作　　者：王利民[1] 沈文律[2] 周珍[3] 张士莲 

机构地区：[1]唐山工人医院普通外科,河北省唐山市063000 [2]汕头大学医学院附属第二医院,广东省汕头市515041 [3]华北煤炭医学院,河北省唐山市063000 [4]开滦矿务局医院,河北省唐山市063000

出　　处：《世界华人消化杂志》2005年第23期2803-2805,共3页World Chinese Journal of Digestology

摘　　要：目的:介绍一种有效鉴别纳米细菌的金标记免疫电镜方法方法:纳米细菌经25 g／L戊二醛固定、树脂包埋后,用钻石刀制成超薄切片,用纳米细菌特异性抗体进行间接金标免疫电镜染色．结果:纳米细菌经负染后于透射电镜下观察,呈球形或短棒状颗粒,大小约为80-350 nm．纳米细菌可以和特异性抗体结合,在电镜下表现为胶体金颗粒的附着．结论:采用免疫电镜的方法,在较好地显示纳米细菌超微结构的同时,又可以指示其独特的抗原性．AIM： To introduce an effective method of immunogold electron microscopy for the detection of nanobacteria, METHODS： Nanobacteria were fixed in the 25 g/L glutaraldehyde, and then were embedded in resin. After they were cut into ultrathin slices by diamond knife, an indirect immunogold electronic microscopy staining was performed on the nanobacteria using the specific antibody of nanobacteria. RESULTS： The nanobacteria were ball- or stick-like with a length of 80-350 nm under electron microscope, Nanobacteria were combined with the specific antibody. The adhesion of the immune colloidal gold granule was observed under the electronic microscopy, CONCLUSION： The immune electronic microscopy can not only reveal the ultrastructure of nanobacteria, but also indicate its special antigenicity.

关 键 词：纳米细菌 免疫电镜 细菌被膜 

分 类 号：Q93-33[生物学—微生物学]