柚皮甙对血管内皮细胞ROS相关酶类基因表达的影响  

Effects of naringin on the expressions of reactive oxygen species(ROS)-related enzymes in endothelial cells

作　　者：刘虹[1] 左学志[1] 易海维[2] 应晨江[2] 叶晓蕾[3] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030 [2]华中科技大学同济医学院营养与食品卫生学系,武汉430030 [3]温州医学院检验与公共卫生学院,温州325035

出　　处：《华中医学杂志》2005年第5期313-314,共2页Central China Medical Journal

基　　金：国家自然科学基金资助项目(No.30471461);湖北省卫生厅科研基金资助项目(No.JX1A01)

摘　　要：目的观察黄酮类物质柚皮甙对血管内皮细胞活性氧族(ROS)产生和清除酶类水平的影响,探讨其抗氧化作用机制。方法在牛颈动脉内皮细胞(BCAECs)培养基质中加入不同浓度的柚皮甙,用免疫印迹检测eNOS(内皮型一氧化氮合酶)、SOD1(超氧化物歧化酶1)、过氧化氢酶、NADPH氧化酶p22phox亚基基因表达的变化。结果0.01～100μmol/L柚皮甙可以明显上调eNOS基因和NADPH氧化酶p22phox亚基基因表达,并且在0.01～1.0μmol/L范围内对eNOS基因表达存在剂量效应关系。结论柚皮甙可以同时上调产生一氧化氮和超氧阴离子的酶蛋白水平,这种作用在血管内皮细胞功能调节中的作用,有待进一步研究。Objective To observe the effects of a flavonoid substance naringin on the expressions of ROS-related enzymes in endothelial cells and explore its anti-oxidation mechanism. Methods 0. 01 - 100 μmol/L naringins were co-incubated with bovine carotid artery endothelial cells for 24 h and the proteins of eNOS, SOD-1, catalase, and NADPH oxidase p22phox subunit were detected by Western blotting. Results The expressions of eNOS and p22phox subunit were up-regulated and have doseeffect relationship on the range of 0. 01 1.0μmol/L naringin. Conclusion Naringin can up-regulate the level of some ROS-related enzyme and further study will be needed to explore the detail effects of naringin on vascular endothelial cells.

关 键 词：一氧化氮合酶 NADPH氧化酶 基因表达 血管内皮细胞 柚皮甙 

分 类 号：R285[医药卫生—中药学]