Molecular cloning and chracterization of a c-Rel induced lectin-like receptor and its alternative splice isoforms  

作　　者：HUI LING BAI YUAN FANG MA WEN ZHI TIAN HSIOU-CHI LIOU 

机构地区：[1]Department of Cellular and Molecular Immunology, Henan University School of Medicine, Kaifeng,R. China [2]Research Institute of Immunology of Henan University, kaifeng, P. R. China [3]Division of Immunology, Department of Medicine, WeiU Medical College of CorneU University, NewYork, NY 10021, USA

出　　处：《Journal of Microbiology and Immunology》2005年第4期280-287,共8页中华微生物学和免疫学（英文版）

摘　　要：The NF-κB family member, c-Rel plays a critical role in the regulation of immune function. It was found that mice with c-Rel deficiency exhibited extensive defects in the survival of lymphocytes and cell cycle progression, and were tolerant to allografts. To further characterize the regulatory function of c- Rel, a representational difference analysis （RDA） was performed on mRNAs derived from B lymphocytes of wild type and the c-Rel knockout mice. By using this approach, a novel gene designated as lympho- cyte-derived C-type lectin-1 （LCL-1） was identified, whose expression was dependent on the intact c-Rel molecule. In the present study, LCL-1 was demonstrated to be a type Ⅱ transmembrane protein with a single extracellular carbohydrate recognition domain （CRD） that exhibited significant degree of homology to C-type lectin-like receptors, including CD69. This LCL-1 gene was found to be located at the NK gene complex （NKC） on mouse chromosome 6 and could encode at least 4 alternatively spliced isofonns. In addition to its expression on B lymphocytes, it was also expressed on immature as well as mature dendritic cells （DCs）, especially with higher expression level on mature DCs. Together, our findings explore one new member of the NKC family and demonstrate for the first time that a lectin-like receptor of the the NKC family is the target gene of c-Rel.The NF-κB family member, c-Rel plays a critical role in the regulation of immune function. It was found that mice with c-Rel deficiency exhibited extensive defects in the survival of lymphocytes and cell cycle progression, and were tolerant to allografts. To further characterize the regulatory function of c-Rel, a representational difference analysis (RDA) was performed on mRNAs derived from B lymphocytes of wild type and the c-Rel knockout mice. By using this approach, a novel gene designated as lymphocyte-derived C-type lectin-1 (LCL-1) was identified, whose expression was dependent on the intact c-Rel molecule. In the present study, LCL-1 was demonstrated to be a type Ⅱ transmembrane protein with a single extracellular carbohydrate recognition domain (CRD) that exhibited significant degree of homology to C-type lectin-like receptors, including CD69. This LCL-1 gene was found to be located at the NK gene complex (NKC) on mouse chromosome 6 and could encode at least 4 alternatively spliced isoforms. In addition to its expression on B lymphocytes, it was also expressed on immature as well as mature dendritic cells (DCs), especially with higher expression level on mature DCs. Together, our findings explore one new member of the NKC family and demonstrate for the first time that a lectin-like receptor of the the NKC family is the target gene of c-Rel.

关 键 词：C-type lectin NF-κB NK gene complex 

分 类 号：Q78[生物学—分子生物学]