稻田土壤固氮微生物的多态性研究方法进展  

Research progress in the diversity of nitrogen fixing microorganism in rice paddy soil

作　　者：罗青[1] 宋亚娜[1] 郑伟文[1] 

机构地区：[1]福建省农业科学院生物技术研究所

出　　处：《福建农业学报》2005年第B12期149-153,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目[闽科(2003)61号]

摘　　要：稻田土壤固氮微生物群落的地理分布和多样性,为生物固氮在农业实践中的高效应用提供了有力证据。实验室中传统的微生物分离、培养和分类方法,在反映土壤微生物的基因信息上有很大的局限,因此目前逐渐地被分子生物学的方法替代。PCR(聚合酶链式反应)-DGGE(变性梯度凝胶电泳)技术能够很好地分离PCR产物中的具体基因,为在时间与空间上追踪优势菌株提供了新的方法。该文以PCR-DGGE技术为主,阐述了利用分子生物学技术研究稻田土壤固氮微生物多样性的基本原理及研究进展。The diversity and geographical distribution of nitrogen fixing microorganism community in rice paddy soils offer a scientific evidence for efficient application of N2 - fixing organism in agricultural practice. Traditional isolation, culture and classification methods for microbe in lab have now been found to have limitation in reflecting the whole genome information of the soil mierobes and have been gradually replaced by molecular biological approaches, PCR （Polymerase Chain Reaetion） -DGGE （Denaturing Gradient Gel Electrophoresis） has been showed to be a powerful tool for separating specific genes from a PCR product and providing a culture-independent method for tracking dominant baterial populations in space and time. This paper presents the basic principle and utilization of molecular biologieal techniques on diversity analysis of N2 -fixing microbe in paddy field with emphasis on PCR - DGGE.

关 键 词：稻田土壤 固氮微生物 多态性 DGGE 

分 类 号：S154[农业科学—土壤学] Q7[农业科学—农业基础科学]