低密度脂蛋白受体基因多态性及其与慢性丙型肝炎的相关性  

Association between polymorphism of human low-density lipoprotein receptor gene and chronic viral hepatitis C in Chinese patients

作　　者：李晗[1] 刘正稳[1] 韩群英[1] 李雁[1] 

机构地区：[1]西安交通大学医学院第一附属医院传染科,710061

出　　处：《中国药物与临床》2006年第2期88-91,共4页Chinese Remedies & Clinics

基　　金：国家自然科学基金资助项目(30170842)

摘　　要：目的探讨我国汉族正常人群及慢性丙型肝炎(chronicviralhepatitisC,CHC)患者低密度脂蛋白受体(LDL-R)基因外显子12中HincⅡ多态性的分布及其与慢性丙型肝炎之间的关系。方法采用聚合酶链反应结合限制性片段长度多态性(PCR-RFLP)的方法,对84例慢性丙型肝炎病毒(HCV)感染者和72名健康献血员进行LDL-R基因HincⅡ多态性检测。结果在HCV感染组和健康对照组、慢性丙型肝炎组和HCV相关肝硬化组、丙氨酸转氨酶(ALT)正常组和ALT升高组,以及HCVRNA阴性组和HCVRNA阳性组之间,LDL-R基因外显子12HincⅡ多态性分布差异无统计学意义。结论人群LDL-R基因外显子12中存在着HincⅡ多态性位点,但该多态性位点可能与慢性HCV感染无相关性。Objective To detect Hinc Ⅱ polymorphism in low-density lipoprotein receptor（LDL-R） gene,and to study its relation to chronic viral hepatitis C （CHC） in the Han nationality of China.Methods Polymerase chain reaction restriction fragment length polymorphisms（PCR-RFLP） method was used to detect Hinc Ⅱ polymorphism of the LDL-R gene in 84 patients infected with HCV. and 72 healthy blood donors.Results There was no statistically significant differences in Hine Ⅱ polymorphism of the LDL-R gene exon 12 between patients infected with HCV and healthy controls,between patients with chronic viral hepatitis C and those with HCV associated cirrhosis,between patients with normalized serum ALT and those with abnormal ALT level, and between HCV RNA negative and positive patients.Conclusion There is Hinc Ⅱ polymorphism in the LDL-R gene exon 12 in the Han natiancity poputation,but it may not be involved in the chronic viral hepatitis C.

关 键 词：受体 LDL 多态现象 遗传 聚合酶链反应 多态性 限制性片段长度 肝炎 丙型 慢性 

分 类 号：R512.63[医药卫生—内科学]