2型糖尿病大鼠血小板CD62表达与主动脉病变关系的探讨  

A preliminary study on the relationship between platelet CD62 and the aorta lesion in type 2 diabetic rats

作　　者：荣青峰[1] 赵宝珍[1] 刘晓玲[1] 贺洪梅[1] 任桂卿[1] 

机构地区：[1]山西医科大学第二医院内分泌科,太原030001

出　　处：《中国药物与临床》2006年第2期111-114,共4页Chinese Remedies & Clinics

摘　　要：目的探讨血小板CD62表达在2型糖尿病大鼠主动脉病变中的作用。方法Wistar大鼠随机分为正常对照组(A组)、高糖高脂喂养组(B组)、糖尿病组(C组)。分别于胰岛素抵抗期、成模后8周、18周时经光镜和透射电镜观察主动脉形态学改变;应用微机图像处理系统,对主动脉内中膜厚度进行动态测量;应用流式细胞术测定循环血中血小板CD62的表达。应用酶联免疫吸附法检测氧化低密度脂蛋白(OX-LDL)。结果镜下糖尿病大鼠主动脉病变出现于成模后8周,但在胰岛素抵抗期已有血小板CD62的表达明显增加,C组始终高于其他两组,而B组则高于A组(P<0.05);主动脉内-中膜厚度与血小板CD62表达和OX-LDL呈显著正相关(r=0.73,r=0.65,P<0.05),与血糖无明显相关。结论2型糖尿病大鼠血小板CD62过度表达先于大血管病变,可能是导致糖尿病大血管病变的重要因素之一,其可作为预测2型糖尿病大血管并发症的早期指标。Objective To explore the role of platelet CD62 in the development of aorta lesion in type 2 diabetic rats. Methods Wistar rats were randomly divided into normal control group （A） ,enriching-fat-diet feeding control group（B） and diabetes control group（C）.At 4,14 and 24 weeks,the early changes of aorta were examined using light microscope and transmission electron microscope. Computorized image processing was employed to measure the aorta intima-media thickness （IMT）.Flow cytometric analysis was applied to detect the expression of CD62 on the surface of platelet isolated from the systemic circulation.Plasma OX-LDL concentration was measured by ELISA. Results The early stages of aorta lesion occurred in the diabetic rats at 14 weeks.The increased expression of CD62 was observed at 4 weeks,and C group was consistendy greater than A and B group,while B group greater than A （P〈 0.05）.The aorta intima-media thickness was correlated positively with the expression of CD62 and OX-LDL （r=0.73, r=0.65,P〈0.05） ,but was not correlated with blood glacose. Condusion The increased expression of CD62 occurred before visible aorta pathological changes,and CD62 may play an important role in the progression of diabetic aorta lesion.It can be used as an early figure to predict diabetic aorta lesion.

关 键 词：糖尿病 2型 大鼠 血小板 CD62 主动脉 

分 类 号：R587.2[医药卫生—内分泌]