人参皂苷对体外培养低氧海马神经元的保护作用  

PROTECTIVE EFFECTS OF Rg_2 ON ANOXIC CULTURED HIPPOCAMPAL NEURONAL CELLS IN NEWBORN RATS IN VITRO

作　　者：王双燕[1] 王守彪[1] 沈若武[1] 李安庆[2] 杨学伟[3] 

机构地区：[1]青岛大学医学院人体解剖教研室,山东青岛266021 [2]淄博市临淄区人民医院消化内科 [3]青岛大学医学院脑血管病研究所

出　　处：《青岛大学医学院学报》2006年第1期42-43,46,共3页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的 观察人参皂苷（Rg2）对大鼠低氧海马神经元的保护作用及其机制。方法 取新生Wistar大鼠海马神经元体外培养14d，随机分为对照组、5μmol/L尼莫地平组（尼莫地平组）、Rg2 0．025mmol/L组（Rg2 1组）、Rg2 0．050mmol/L组（Rg2 2组）。将相应药物加入到培养液中孵育4h后，连续充以体积分数0．95N2＋体积分数0．05CO2混合气体，建立急性低氧细胞模型。台盼蓝染色计数存活细胞，吉姆萨染色观察细胞形态，荧光分光光度计法测细胞内Ca^2＋浓度。结果 尼莫地平组、Rg2两个剂量组的细胞死亡率均明显低于对照组，以Rg2 2组最低，差异均有显著性（X^2=3．37，P〈0．05）。海马神经元细胞内Ca^2＋浓度在尼莫地平组、Rg2两个剂量组均明显低于对照组，以Rg2 2组最低，差异均有显著性（F=466．58，q=6．76～48．82，P〈0．01）。结论 Rg2可显著降低体外培养大鼠低氧海马神经元的细胞死亡率，其机制可能是通过减少Ca^2＋内流而发挥作用。Objective To investigate the neuroprotective effect and its mechanism of Rg2 in anoxic cultured hippocampal neuronal cells in newborn rats. Methods Anoxic cultured hippocampal neuronal ceils of newborn rats were divided into control group, nimodipine group （5 μmol/L）, Rg2 1 （0. 025 mmol/L） group and Rgz 2 （0. 050 mmol/L） group. The activities of the neurons were tested by trypan blue staining. The morphological changes were observed by Giemsa staining. The concentration of cellular free calcium in the cells was tested by Fluorescence spectrophotometer （Fura-2）. Results In vitro, compared with the control group, nimodipine and Rg2 significantly increased the activities of hippocampal neuronal ceils, decreased the apoptosis rate and the concentration of cellular free calcium in the cells, and the most effective in Rg2 0.050 mmol/L group. Conclusion The Rg2 could protect hippocampal neuronal cells against anoxia by decreasing the concentration of cellular free calcium in the cells to inhibit the cell death.

关 键 词：人参皂苷 缺氧 脑 海马 神经元 钙 

分 类 号：R285[医药卫生—中药学]