系统性红斑狼疮患者树突状细胞亚型水平与疾病活动指数的相关性  

Correlation between levels of dendritic cell subsets in systemic lupus erythematosus patients and SLEDAI

作　　者：任莉莉 齐晖 李富荣 刘冬舟 肖学吕 王新根 黄瑞芳 文锦丽 

机构地区：[1]深圳市人民医院(暨南大学第二临床医学院)临床医学研究中心,广东深圳518001 [2]深圳市人民医院(暨南大学第二临床医学院)风湿科,广东深圳518001

出　　处：《中国现代医学杂志》2006年第4期543-545,共3页China Journal of Modern Medicine

基　　金：广东省自然科学基金资助项目(021339)

摘　　要：目的检测系统性红斑狼疮患者树突状细胞亚型水平,探讨与疾病活动指数的相关性。方法使用免疫荧光染色、流式细胞仪检测髓系树突状细胞(MDC)亚型CD11c+和淋巴系树突状细胞(PDC)亚型CD123+的表达,并与SLEDAI、ds-DNA、C3、C4进行相关性分析。结果显示SLE患者和活动期患者外周血中CD11c+和CD123+表达(0.21±0.12);(0.19±0.13)较正常对照组(0.36±0.07)和(0.29±0.9)明显降低(P<0.05)。在SLE病人中,活动期组(0.16±0.11);(0.13±0.06)和静止期组(0.26±0.13);(0.23±0.16)患者外周血CD11c+和CD123+百分率差异存在显著性(P<0.05)。CD11c+和CD123+水平与SLEDAI、ds-DNA具有明显相关性(r=-0.408、-0.436、-0.474、-0.528;P分别为0.018、0.013、0.009和0.003),与C3、C4无明显相关性。结论SLE患者外周血树突状细胞亚型明显降低,并且与SLEDAI相关明显,可作为观察狼疮活动的客观指标。[Objective] Detect the levels of dendritic cell subsets in systemic lupus erythematosus （SLE） patients, and study its correlation with SLE activity criteria. [Methods] The immunofluorescence staining and flow cytometer were used to measure the expression of the myeloid dendritic cell （MDC） subsets CD11c＋ and the plasmacytoid dendritic cell （PDC） subsets CD123＋, and the correlations with SLEDAI, ds-DNA, C3 and CA were analyzed. [Results] The expression of CD 11 c＋ and CD 123＋ as （（3.21 ±0.12）; （（3.19 ±0.13） in peripheral blood of SLE patients and active phase patients decreased significantly （P 〈0.05） compared with control groups （0.36±0.07）; （0.29±0.9）. There were significant differences （P 〈0.05） of the percents of peripheral blood CD11c＋ and CD123＋ between active phase SLE groups as （0.16±0.11）; （0.13±0.06） or quiescent period SLE groups as （0.26±0.13）; （0.23±0,16）. The levels of CD11c＋ and CD123＋ were correlated significantly with SLEDAI and ds-DNA （with r value of -0.408, -0.436, - 0.474, -0.528, and p of 0.018, 0.013, 0.009 and 0.003 respectively）, but had no evident correlation with C3 and C4. [Conclusions] The results indicated that peripheral blood dendritic cell subsets decreased significantly in SLE patients, and could be used as an impersonal SLE activity index.

关 键 词：树突状细胞 系统性 红斑狼疮 疾病活动指数 

分 类 号：R593.2[医药卫生—内科学]