用流式细胞术研究PKC及VRP，CsA对多药抗性细胞的调制与逆转  

STUDIES WITH FLOW CYTOMETRY ON THE REGULATION OF PKC AND REVERSAL EFFECTS OF VRP,CsA ON MULTIDRUG RESISTANT CELL LINES

作　　者：周卫东[1] 张鸿卿[1] 方敏[1] 薛绍白[1] 

机构地区：[1]北京师范大学生物学系

出　　处：《北京师范大学学报（自然科学版）》1996年第1期129-133,共5页Journal of Beijing Normal University(Natural Science)

基　　金：国家自然科学基金;国家教委博士点基金

摘　　要：利用流式细胞术检测抗药性细胞内Ｒｈｏ－１２３的荧光强度作为检测抗性细胞抗性程度的指标，研究了蛋白激酶Ｃ（ＰＫＣ）抑制剂Ｈ７，肿瘤促进剂佛波酯（ＴＰＡ）对多药抗性细胞的调节；钙离子通道阻断剂维拉帕米（ＶＲＰ），免疫抑制剂（ＣｓＡ）对多药抗性的逆转作用．测定了抗性细胞细胞膜和胞浆ＰＫＣ活性水平，指出ＰＫＣ可能通过磷酸化细胞膜上的Ｐ－糖蛋白（Ｐ－ｇｐ）来调节多药抗性细胞的抗性水平，并提供了一种筛选多药抗性逆转药物的简便方法．Flow cytometry was used to detected Rho-123 fluorescence intensity, as a mark of multidrug resistance. Through this approach, the regulation of PKC inhibitor H7 and tumor promotive agent phorbol ester (TPA) on multidrug resistance, the reversal effects of calcium channal blocker verapamil and immunosuppression agent cyclosporin (CsA) to the multidrug resistant cell lines were studied. The PKC activity, it was suggested that PKC regulate multidrug resistance by means of phosphorylating P-gp is also measured. A kind of simple method which would be used to select multidrug resistance reversal drugs was proposed.

关 键 词：流式细胞术 多药抗性 蛋白激酶 VRP 

分 类 号：R963[医药卫生—微生物与生化药学]