细粒棘球蚴中国大陆株Eg10基因片段的分子克隆和序列分析  

Cloning And Sequence Analyzing of the Eg10 Gene on Echinococcus Granulosus from China

作　　者：张炜[1] 王娅娜[2] 丁淑琴[2] 王健[2] 赵巍[2] 杨玉荣[1] 

机构地区：[1]宁夏医学院人体寄生虫学教研室,银川750004 [2]宁夏医学院医学遗传学与细胞生物学教研室,银川750004

出　　处：《宁夏医学院学报》2006年第1期1-3,16,共4页Journal of Ningxia Medical College

基　　金：国家自然科学基金(30260105);宁夏自然科学基金(NZ0540);宁夏卫生厅重点计划项目(2002)

摘　　要：目的获得细粒棘球蚴(E.granulosus)Eg10基因片段,并进行序列分析。方法从包虫病患者体内获取细粒棘球蚴原头蚴,提取总RNA,根据GeneBank公布的细粒棘球蚴Eg10基因片段已知序列,设计一对引物,采用RT-PCR技术扩增出细粒棘球蚴中国大陆株Eg10基因片段,将纯化后的PCR产物克隆到pGEM-T载体,进行序列测定和分析。结果成功扩增出细粒棘球蚴中国大陆株Eg10基因片段,测序为938bp,该基因序列与检索基因核苷酸序列同源性为100%,推导编码氨基酸序列同源性亦为100%。结论测序的Eg10基因序列有完整的编码框(765bp),对于进一步研究其结构和功能及对包虫病的免疫预防具有重要意义。Objective To obtain the Eg10 gene of echinococcus granulosus and analyze its sequence. Methods Total RNA was extractedfrom protoscoleces of Echinococcus granulosus of humam origin. The specific primers were designed according to published nucleotide sequence in Genebank. The Eg10 gene fragment was amplified By RT- PCR and then cloned it into pGEM - T vector for sequencing and analyzing. Results The Eg10 sequence was successfully amplified, which was 938bp. The sequence and its amino acid sequence showed 100% homology with that published in Genebank previously. The deduced sequence of coding amino acid was 100% homology, too. Conclusion The sequenced Eg10 gene has a completely open coding frame （765bp）. It will be significant to study its structure and function, as well as immunizing prevention.

关 键 词：细粒棘球蚴 Eg10 分子克隆 序列分析 

分 类 号：R383[医药卫生—医学寄生虫学]