An Atomic Force Microscopy Study on Small Unilamellar Vesicle Structures on Mica  

作　　者：李学丰 张高勇 董金凤 周晓海 洪昕林 

机构地区：[1]Department of Chemistry, Wuhan University, Wuhan, Hubei 430072, China

出　　处：《Chinese Journal of Chemistry》2006年第3期311-315,共5页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （No. 20573079）, The authors are grateful to Prof. Daiwen PANG and Dr. Zhexue LU of Department of Chemistry of Wuhan University, Wuhan, for their kindly help in the AFM measurements.

摘　　要：The vesicle structures of egg yolks phosphatidylcholine/didodecyldimethylammonium bromide （1 ： 1, mass ratio） deposited on mica were studied by atomic force microscopy （AFM） both in aqueous phase and air. In aqueous phase both bilayer and domelike vesicles with a mean diameter of 45 nm were observed, whereas in air the structure was more complicated depending on the initial concentration of vesicles. Vesicles with the original size could only be visualized at very low concentration with a mean diameter of 55 nm, which is a little larger than the result obtained in aqueous phase. At higher concentrations, fused large aggregates and multiple bilayer with a thickness ca. 4 nm of each bilayer were dominated. A plausible adsorption mechanism was proposed based on the experimental results.The vesicle structures of egg yolks phosphatidylcholine/didodecyldimethylammonium bromide （1 ： 1, mass ratio） deposited on mica were studied by atomic force microscopy （AFM） both in aqueous phase and air. In aqueous phase both bilayer and domelike vesicles with a mean diameter of 45 nm were observed, whereas in air the structure was more complicated depending on the initial concentration of vesicles. Vesicles with the original size could only be visualized at very low concentration with a mean diameter of 55 nm, which is a little larger than the result obtained in aqueous phase. At higher concentrations, fused large aggregates and multiple bilayer with a thickness ca. 4 nm of each bilayer were dominated. A plausible adsorption mechanism was proposed based on the experimental results.

关 键 词：egg yolks phosphatidylcholine didodecyldimethylammonium bromide small unilamellar vesicle BILAYER atomic force microscopy 

分 类 号：Q545.1[生物学—生物化学]