睫状神经营养因子对面神经损伤的修复作用  

Ciliary neurotrophic factor in the regeneration of facial nerve

作　　者：张治军[1] 高炳庆[2] 梁传余[2] 廖志钢[3] 徐建国[4] 刘亚峰[2] 

机构地区：[1]上海中医药大学附属曙光医院耳鼻咽喉科,上海200021 [2]四川大学华西医院耳鼻咽喉科,四川成都610041 [3]四川大学基础医学与法医学院法医学教研室,四川成都610041 [4]四川大学基础医学与法医学院生理学教研室,四川成都610041

出　　处：《中国耳鼻咽喉头颈外科》2006年第2期92-94,共3页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的探讨局部应用睫状神经营养因子对面神经损伤的修复作用。方法将成年新西兰兔面神经上颊支切除5mm,在断端间置入硅胶再生室,实验组室内注入睫状神经营养因子,对照组用生理盐水。术后4周和8周进行电生理学、组织病理学观察和形态学定量分析。结果术后4周,两组电刺激面神经很少能引发面肌兴奋,有髓轴索计数,轴索直径和面积t检验,两组差异有显著性意义(P<0.05)。术后8周面神经复合肌动作电位,实验组运动神经传导速度明显短于对照组,有髓轴索计数,轴索直径和面积实验组大于对照组(P<0.05)。结论局部应用睫状神经营养因子可促进面神经损伤的修复。OBJECTIVE To study the role of ciliary neurotrophic factor（CNTF） in the generation of facial nerve.METHODS Twenty adult New Zealand rabbits＇ bilateral super buccals of facial nerves were transected and connected with silicone tube.CNTF was injected into a random side of silicone tube and normal saline in the other.At four and eight weeks after the operation, both the CNTF group and the SAL group underwent electrophysiology test and histopathology as well as quantity analysis.RESULTS Four weeks later, both CNTF and SAL group failed in eliciting muscular excitement upon stimulation; T-test showed a significant difference （P〈0.05） in the average number of myelinated axons, the axon cross-sectional areas and the diameter of axons in the two groups.Eight weeks later, motor nerve conduction velocity of facial muscular excitement was 37.9 ± 4.4m/s in the CNTF group and 30.5 ± 4.6m/s in the SAL group.The average number of myelinated axons,axon cross-sectional areas and diameter of axons were 1914 ± 475, 2.17 ± 0.21, 3.12 ± 0.51 in the CNTF group and 1197 ± 403, 1.86 ± 0.28, 2.79 ± 0.62 in the control one,respectively.T-test showed a significant difference between them （P 〈0.05）.CONCLUSION CNTF enhances facial nerve regeneration in adult New Zealand rabbits.

关 键 词：睫状神经营养因子 面神经损伤 神经再生 

分 类 号：R651.3[医药卫生—外科学] R587.2[医药卫生—临床医学]