Cloning and bioinformatic analysis of HSPC016 gene in dermal papilla cells  

作　　者：王继文 宋志强 邓永键 郝飞 

机构地区：[1]Department of DermatologySouthwest Hospital,Third Military Medical University,Chongqing 400038,China

出　　处：《Journal of Medical Colleges of PLA(China)》2006年第1期7-12,共6页中国人民解放军军医大学学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No.30200249)

摘　　要：Objective： To clone the full-length cDNA sequence of HSPC016 gene, an aggregative growth related gene in dermal papilla cells （DPC）, and analyze its characteristics and predict its biological function. Methods： Rapid amplification of cDNA ends （RACE） technology was entailed to amplify the 5＇ and 3＇ sequences of HSPC016. The amplified fragments were TA-cloned, sequenced and spliced together to obtain the full-length cDNA. Its chromosome localization, domain and possible function were analyzed by bioinformatic methods. Results.. Two isoforms, 400 bp and 493 bp, were obtained. The gene was mapped on chromosome 3q21. 31, and was conservative on evolution. HSPC016, a 64aa protein, belongs to PD053992 protein family and its functional domain was homologous to T2FA gene. Conclusion： PISPC016 may be related to transcriptional regulation and its protein product may act as a subunit of a transcriptional complex and play a role on DPC growth and differentiation through facilitating or suppressing other genes＇ transcription within the nucleus.

关 键 词：dermal papilla cells HSPC016 transcriptional regulation 

分 类 号：R346[医药卫生—基础医学]