中药狼疮方对狼疮样BXSB小鼠肺组织CD134/CD134L和RANTES表达的影响  

Effects of lupus recipe on gene expressions of CD134,CD134L,and RANTES in pulmonary tissue of lupus-prone BXSB mice

作　　者：周燕斌[1] 叶任高[1] 李幼姬[1] 谢灿茂[1] 胡斌[2] 

机构地区：[1]广州中山大学附属第一医院内科,广州510080 [2]广州中山大学达安基因中心,广州510080

出　　处：《免疫学杂志》2006年第2期165-167,171,共4页Immunological Journal

摘　　要：目的探讨中药狼疮方对狼疮样BXSB小鼠肺组织CD134/CD134L和RANTES表达的影响。方法采用BXSB小鼠模型,随机分为3组:狼疮方治疗组、强的松治疗组、未治疗组,每组6只,疗程10周。另设与BXSB小鼠同基因的正常C57BL/6小鼠6只为正常对照组。分别取小鼠肺组织,应用逆转录-荧光定量-聚合酶链反应(RT-FQ-PCR)技术定量测定小鼠肺组织CD134、CD134L和趋化因子RANTES的mRNA表达水平。结果①未治疗组小鼠肺组织CD134、CD134L mRNA和RANTESmRNA的表达水平都显著高于正常对照组(P<0.01,P<0.05);经强的松或中药狼疮方治疗后,BXSB小鼠肺组织CD134、CD134L及RANTES的mRNA表达都受到明显抑制,显著低于未治疗组(P<0.01,P<0.05);且接近正常水平,与正常对照组无显著性差异(P>0.05)。②BXSB小鼠肺组织RANTES的mRNA表达水平与CD134L的mRNA表达水平呈显著的正相关关系(r=0.793,P<0.05),而与CD134的mRNA表达水平无显著的相关关系(r=0.412,P>0.05)。结论中药狼疮方具有与强的松类似的免疫抑制作用,可显著抑制狼疮样小鼠肺组织CD134/CD134L共刺激信号表达;并下调肺组织RANTES mRNA表达水平,具有一定的肺脏保护作用。Objective To investigate the effects of lupus recipe on gene expressions of CD134, CD134L, and RANTES in lung cells of lupus-prone BXSB mice. Methods Eighteen male lupus-prune BXSB mice models were divided into three groups： untreated group, lupus recipe （LR） treated group,and prednisone treated group. All mice were sacrificed in 10 weeks. Control group was set up which consisted of 6 normal syngeneic C57BL/6 male mice. The mRNA expressions of CD134, CD134L, and RANTES in lung cells were detected by RT-FQ-PCR. Results ① The mRNA expressions of CD134, CD134L, and RANTES in lung cells of untreated group were obviously higher than those of control groups （P 〈 0.05, P 〈 0.01）. The LR or prednisone reduced significantly the CD134, CD134L, and RANTES mRNA expressions （P 〈 0.05, P 〈 0.01, comparing with untreated group）, while the difference between the treated group and the control group wns not significant. ② There was a positive correlation between CD134L mRNA expression and RANTES mRNA expression in lung cells of model mice （r = 0.793, P 〈 0.05）. Conclusion The abnormal expressions of CD134, CD134L mRNA are well evidenced in the lungoflupus-prone BXSB mice. Strong expression of RANTES mRNA is likely mediated by CD134/CD134L signal pathway. LR and corticusteroide could obviously inhibit the mRNA expressions of CD134, CD134L, and RANTES.

关 键 词：系统性红斑狼疮 狼疮方 CD134 CD134L RANTES 

分 类 号：R285.5[医药卫生—中药学]