MUC1黏蛋白模拟表位的筛选和鉴定  

Selection and identification of MUC1 mimic epitope

作　　者：崔志刚[1] 宋波[1] 张立新[2] 路浩军[2] 李春海[2] 

机构地区：[1]第三军医大学西南医院泌尿外科,重庆400038 [2]军事医学科学院附属医院肿瘤分子生物学实验室,北京100850

出　　处：《免疫学杂志》2006年第2期210-212,共3页Immunological Journal

基　　金："863"课题资助项目(2002AA214111)

摘　　要：目的从噬菌体表面展示的随机12肽库中筛选出MUC1抗原的模拟表位。方法通过生物淘洗,获得阳性噬菌体克隆,通过基因测序,推导氨基酸序列,同MUC1核心序列比较,选出2个模拟表位,进行抗原表位预测和竞争抑制实验。结果筛选到的两个模拟表位与MUC1单克隆抗体的亲和力均较强,能特异性抑制MUC1的抗原抗体结合,且包含有与某些MHCⅠ类分子较好结合的位点。结论从噬菌体12肽库中筛选出两个MUC1的模拟表位可以作为靶向MUC1肿瘤疫苗的候选肽。Objective To semen two MUC1 antigen mimic epitopes by phage display peptide library technology. Methods Two positive phage clones were obtained by biopanning, then their DNA sequences were determined and amino acid sequences were deducted. By comparing with MUC1 core sequence, two MUC1 mimic epitopes were pick out. The two mimic epitopes were tested by competitive inhibition test and prediction test. Results The two MUC1 mimic epitopes were contained MHCⅠ binding sites, and could specifically combine MUC1 moroclonal antibody as well as inhibit the binding between MUC1 antigen and its monoclonal antibody. Conclusion on The two MUC1 mimic epitopes obtained from phage displayed twelve peptide libraries can be used as suitable guide peptide for the development of tumor vaccines targeting MUC1.

关 键 词：MUC1 噬菌体表面展示 模拟表位 

分 类 号：R346[医药卫生—基础医学]