形成素结合蛋白C-端FF结构域的~1H和^(15)N NMR谱峰归属和结构分析  

Assignment of ~1H and ^(15)N Chemical Shifts and Structure Elucidation of C-terminal FF-domain of the Formin-Binding Protein

作　　者：吴昌琳[1] 宋国强[2] 胡红雨[3] 

机构地区：[1]华东师范大学物理系 [2]中国科学院上海药物研究所,上海201203 [3]中国科学院上海生物化学和细胞生物学研究所,上海200031

出　　处：《波谱学杂志》2006年第1期1-10,共10页Chinese Journal of Magnetic Resonance

摘　　要：FF结构域广泛存在于与信号传导相关的蛋白质中,我们选择形成素结合蛋白11(forminbindingprotein11,FBP11)中所含的一个FF结构域,通过在H2O和D2O中采集的COSY,TOCSY,NOESY等二维核磁共振谱,识别了FF结构域的氨基酸残基的质子自旋系统,通过解析二维和三维NOESY谱中的dαN、dNN和dβN等NOE相关完成了序列专一性归属;二级结构分析表明FF结构域包含三段α螺旋.FF-domains have been found widely in many signaling proteins. In this paper, the structure characteristics of FF-domain of the formin-binding protein 11 （FBP11） were studied by NMR spectroscopy. The spin systems for most of the amino acid residues in the FF-domain were identified by analyzing NMR spectra acquired in H2O and D2O. Sequence specific assignment of the spin systems was made using daN, dNN and dβN NOEs correlation calculated from NOESY spectra. The secondary structure of the FF- domain was found to include three a helices.

关 键 词：核磁共振 FF-结构域 序列专一归属 二级结构 

分 类 号：O641[理学—物理化学]