小鼠巨噬细胞内游离钙离子变化介导的吞噬作用(英文)  

Cytosolic Free Clcium Fluctuation Mediates Phagocytosis in Mouse Macrophages

作　　者：林珏龙[1] 陈耀文[1] 谢仰民[2] 李玫[2] 朴仲贤[1] 杨海伟[1] 

机构地区：[1]汕头大学医学院中心实验室 [2]汕头大学医学院动物中心,广东汕头515031

出　　处：《激光生物学报》2006年第1期65-72,共8页Acta Laser Biology Sinica

摘　　要：为评估小鼠巨噬细胞吞噬死亡细胞时胞质内游离钙离子的变化。实验使用F luo-3标记巨噬细胞内钙离子和碘化丙碇对死亡细胞核染色,观察吞噬过程中细胞内钙离子的变化和显示巨噬细胞的吞噬功能,检测含死亡细胞的巨噬细胞内荧光密度图像。利用激光扫描共聚焦显微镜检测钙离子的释放。在缺钙的溶液中,可见巨噬细胞接触死细胞时细胞内钙离子快速地聚集和增高。在吞噬体形成时,巨噬细胞内钙离子上升到较高的水平。快速上升后,当吞噬小泡消化时,细胞内游离钙下降,随后钙离子恢复到低水平。研究显示伴随着吞噬小泡中红色荧光的死细胞的出现和消失,巨噬细胞内出现一系列钙离子变化的图像。提示巨噬细胞内钙离子改变在细胞吞噬作用中具有一定的作用。Cytosolic free calciu, m （ [ Ca^2＋ ] i ） change was assessed in single adherent mouse macrophages during phagocytosis for dead cells. To determine visualizing Ca^2＋ change during phagocytosis and indirectly show phagocytic function of macrophages to dead cells, intracellular [ Ca^2＋ ] i of macrophages were loaded with the fluorescent dye Fluo3/AM and dead cells were labeled with Propidium Iodide（ PI）, we examined images pattern of the fluorescence intensity in macrophages of containing dead cells. A discharge of calcium was verified by confocal scanning laser microscopy （ CSLM ）. In lacking Ca^2＋ medium, upon contact with dead cells, rapid assemble and increase of[ Ca^2＋ ] i was observed in macrophage. Intracellular [ Ca^2＋ ]i rose to a higher level during initial phagosome formation. After a rapid rise, free Ca^2＋ was decreased in digestion of phagocytotic vesicle; there-after the [ Ca^2＋ ]i returned to the baseline. Our study directly indicates that accompany presenting and disappearing of dead cell of red fluorescence in the phagosomal vesicle, a series of images pattern of [ Ca^2＋ ] i change can be really exhibited in macrophages. It is suggested that [ Ca^2＋ ] i fluctuation has a certainty in macrophages during the phagocytosis .

关 键 词：钙离子 脱氧核糖核酸 巨噬细胞 吞噬作用 激光扫描共聚焦显微镜 

分 类 号：Q631[生物学—生物物理学] R318.51[医药卫生—生物医学工程]