Identification of a bi-directional promoter from Tomato yellow leaf curl China virus  

作　　者：GUAN Cuiping ZHOU Xueping 

机构地区：[1]Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China

出　　处：《Progress in Natural Science:Materials International》2006年第3期281-286,共6页自然科学进展·国际材料（英文版）

基　　金：Supported by National Fund for Distinguished Young Scholars (Grant No. 30125032)National Natural Science Foundation of China (Grant No.30530520)

摘　　要：A bi-direetional promoter of Tomato yellow leaf curl China virus （TYLCCNV） was obtained with the total DNA from TYLCCNV isolate Y10 infected tobacco leaves as a template. Plant expression vectors were constructed by fusing the amplified DNA fragment with the gus gene and nopaline terminator in different orientations. The vectors containing promoter fragments were transferred into leaf cells and plant stems of Nicotiana benthamiana by Agrobacteriurn-mediated method. Transient expression results showed that both the complementary and virion-sense promoters could drive the gus gene to express, and the GUS activity of the complementary-sense promoter was stronger than that of the virion sense. Co-expression of the vector containing βC1 gene of TYLCCNV DNAβ with the vector containing a bi directional promoter revealed that the βC1 protein has no impaet on expression of either the virionor the complementarysense promoter.西红柿黄叶卷屈中国病毒(TYCCNV ) 的一个双向倡导者从 TYLCCNV 与全部的 DNA 被获得孤立感染的烟草作为一个模板离开的 Y10。工厂表示向量被在不同取向与 gus 基因和空操作排成直线终止者熔化放大 DNA 碎片构造。包含倡导者碎片 weer 的向量转了在到叶房间和由调停 Agrobacterium 的方法的 Nicotina banthamiana 的工厂 sterms。短暂表达式结果证明 complementery 和 virion 感觉倡导者能驾驶 gusgene 到快车和 complementry 感觉倡导者的 GUS 活动比 virion 感觉的强壮。与包含一个双向倡导者的向量包含 TYLCCNV DNAβ 的 βC1 基因的向量的合作表示表明 βC1 蛋白质没有影响 viron 或互补感觉倡导者的没有表示。

关 键 词：Tomato yellow leaf curl China virus bi-directional promoter transient expression geminivirus. 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]