树突状细胞负载Tα146-162对TAChR预致敏T细胞免疫反应的影响  

The Effect of DC Pulsed with Tα146-162 on Immunoreaction of T Cell Primed with TAChR

作　　者：胡珏[1] 黄颉[1] 李水仙[1] 杨欢[1] 肖波[1] 

机构地区：[1]中南大学湘雅医院神经内科,湖南长沙410008

出　　处：《中国神经免疫学和神经病学杂志》2006年第2期82-85,共4页Chinese Journal of Neuroimmunology and Neurology

基　　金：回国人员科研启动基金资助项目(教外司留2005-383)

摘　　要：目的探讨髓源不成熟的树突状细胞(iMDC)负载Tα146-162在TAChR预致敏小鼠中能否诱导免疫耐受。方法Tα146-162负载于体外培养的iMDC,获得Tα146-162-iMDC。流式细胞术分析iMDC上CD11c、MHC-Ⅱ、CD40和CD86的表达。24只小鼠随机分为A1、A2、K1、K2 4组。第0天,A1组、A2组用TAChR+CFA致敏,K1组、K2组用KLH+CFA致敏。第7天,A2组、K2组注射Tα146-162-iMDC,A1组、K1组则注射PBS。第14天,以3H-TdR掺入法检测淋巴细胞增殖反应。结果培养6 d的iMDC中等程度表达CD11c[(63.94±5.11)%]、低表达MHC-Ⅱ[(39.26±5.81)%]、CD40[(26.60±4.03)%]和CD86[(18.41±3.68)%]。与A1组相比,A2组TAChR刺激下淋巴细胞增殖反应显著降低(P<0.05),ConA和KLH刺激下淋巴细胞增殖反应差异无统计学意义(P>0.05)。K1组与K2组在KLH刺激下淋巴细胞增殖反应差异无统计学意义(P>0.05)。结论Tα146-162-iMDC可在TAChR预致敏的C57BL/6小鼠中诱导免疫耐受。Objective To investigate whether iMDC pulsed with immuno-dominat peptide Tα146-162 can induce tolerance in T cell primed with TAChR. Methods IMDC were cultured in vitro and pulsed with Tα146- 162. Cell surface markers of DC were analysed by flow cytometry. Animals were randomly enrolled into four groups. At day 0, group A1 and A2 were primed with TAChR in CFA and group K1 and K2 were primed with KLH＋CFA. At day 7, group A2 and K2 were injected Tα146-162-iMDC while A1 and K1 group received PBS at the same time. At day 14, the lymphocyte proliferative response of the/our groups were measured. Results IMDC cultured 6 days expressed CD1 lc moderately [（ 63.94±5.11 ）%] and expressed low levels of MHC-Ⅱ[（39. 26±5. 81 ） % 7, CD40 [（ 26.60±4.03 ） % ] and CD86 [- （ 18. 41±3.68 ） % 7. Compared with A1 group, lymphocyte proliferative response to TAChR of A2 group was suppressed significantly （P〈0.05）. No different lymphocyte proliferative response to KLH and ConA were seen in A1 and A2 group （P〉 0. 05）. No different lymphocyte proliferative response were seen in KLH1 and KLH2 groups（P 〉0. 05）. Conclusions Tα146-162 pulsed iMDC can induce tolerance in TAChR primed C57BL/6 mice.

关 键 词：树突状细胞 Tα146—162 免疫耐受 TAChR 

分 类 号：R392[医药卫生—免疫学] R746[医药卫生—基础医学]