大麦品种资源筛选与TrxS基因的转化利用  

Screening of Barley Varieties Resource and Utilization of TrxS Gene

作　　者：孔维威[1] 李磊[1] 卫丽[1] 尹钧[1] 

机构地区：[1]河南农业大学国家小麦工程技术研究中心,河南郑州450002

出　　处：《华北农学报》2006年第1期5-9,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家转基因植物研究与产业化专项(JY03-B-19-03);河南省科技攻关重点项目(0323011900)

摘　　要：对68份大麦种质材料在河南郑州生态条件下进行农艺性状和生态适应性鉴定,从中筛选出适合河南省生态条件的综合农艺性状好的材料31份;利用国家小麦工程技术研究中心获得的转基因大麦新品系,通过有性杂交及回交的方法,将TrxS基因导入41份材料中,经PCR和RT-PCR检测,证明TrxS基因已初步整合进20份材料中,且在部分材料中能够正常表达。本研究获得的材料将为大麦品质育种提供新的种质资源,为分子标记辅助育种提供科学依据。Sixty-eight barley accessions were evaluated in the ecological condition of Henan, and 31 accessions were selected as breeding resources of barley. TrxS gene was transferred into 41 accessions by hybridization and backcross. The integration and transcription of the TrxS gene were confirmed in part of barley lines by PCR and RT - PCR analysis respectively. The results provided new germ plasm for quality breeding of barley and scientific basis for molecular marker assisted breeding.

关 键 词：大麦 品种资源 TRXS基因 分子标记辅助育种 

分 类 号：S512.3[农业科学—作物学]