小麦黄色花叶病毒RNA2自然缺失突变体的筛选和定位分析  

Location of Spontaneously Deleted Region of Wheat Yellow Mosaic Virus RNA2 in Inoculated Wheat

作　　者：张卫华[1] 杨军[1] 韩成贵[1] 李大伟[1] 于嘉林[1] 

机构地区：[1]中国农业大学农业生物技术国家重点实验室和植物病理学系,北京100094

出　　处：《华北农学报》2006年第1期80-85,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家973项目(G2000016201);国家自然基金项目(39870028)

摘　　要：许多真菌介体传播的病毒在机械接种过程中易于发生自然缺失。利用在小麦上连续机械接种WYMV的方法，自第27代起，利用RT-PCR和Northern blot方法在感病小麦中检测到一个WYMV RNA2的缺失突变株，序列分析发现缺失区域位于RNA2的214～2808nt，共计2595nt，并在缺失区域的两端存在7个碱基的反向互补序列。缺失区域上游紧邻这7个碱基双链区存在一富含AU的短序列，并据此对此D-RNA2的缺失机制进行了讨论。Fungus-transmitted plant viruses are liable to naturally delete some internal sequences from their genomes in infected plants during repeated mechanical passages. In this paper, a deleted mutation in RNA2 of WYMV was detected after the 27 passage of mechanical innoculation in wheat by RT-PCR and Northern blot. Sequence analysis showed that a contiguous sequence of 2595 nucleotides （from nt 214 to nt 2808） was internally deleted from WYMV RNA2 and flanked by seven reverse repeated compatible nucleotides which can form a loop-stem structure. A short sequence rich of base A（or U） connect to the left deleted region, which is the deletion signal in many viruses. The possible mechanism of the generation of deleted RNA2 of WYMV is discussed.

关 键 词：WYMV 机械接种 RNA2缺失突变株 

分 类 号：S432.41[农业科学—植物病理学]