不同附加物对甘草愈伤组织培养中黄酮类化合物形成的影响  

Effects of Different Additives on Accumulation of Flavonoids in Glycyrrhiza uralensis Callus

作　　者：杨世海[1] 刘晓峰[2] 果德安[3] 郑俊华[3] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林亚泰(集团)股份有限公司,长春130031 [3]北京大学药学院,北京100083

出　　处：《中国药学杂志》2006年第2期96-99,共4页Chinese Pharmaceutical Journal

摘　　要：目的研究了酵母提取物、水解酪蛋白、真菌诱导子、茉莉酸及稀土元素等对甘草愈伤组织中甘草黄酮类化合物合成的影响。方法在培养基中添加各种附加物,研究其对甘草黄酮类化合物合成的影响。结果 0．1％的酵母提取物可使培养物中黄酮类化合物的含量提高76．8％,甘草查尔酮含量比对照提高7．5倍。水解酪蛋白在0．05％时,培养物中黄酮类化合物含量达最大值,为对照的1．7倍,其中甘草查尔酮的含量在0．1％时最高,为对照的3．2倍。50 mL培养基中加入真菌诱导子2 mL,甘草培养物中黄酮类化合物的含量为149．58μg·g-1,比对照(85．26μg·g-1)增加75％。10．0μmol．L-1茉莉酸使黄酮类化合物的含量比对照提高了41％。低浓度的稀土元素Eu3+对黄酮类化合物的生物合成具有明显的促进作用,培养基中添加0．1 mg·L-1的稀土元素Eu3+最有利于黄酮类化合物的积累。此时,黄酮类化合物的含量为对照的2．7倍,其中甘草素的含量是对照的4倍。结论在甘草愈伤组织培养过程中,添加酵母提取物、水解酪蛋白、真菌诱导子、茉莉酸及稀土元素等是提高黄酮类化合物的有效手段之一。OBJECTIVE To investigate the effects of yeast extract, casein hydmlysate, fungal elicitor, JA and rare-earth element Eu^3＋ on flavonoids production in Glycyrrhiza uralensis callus.METHODS The flavonoids content in Glycurrhiza uralensis callus was analyzed by HPLC after different additives were administrated into culture medium. RESULTS 0.1% yeast extract resulted in 76.8% increase in the flavonoids accumulation, the licochalcone content was 7.5 times higher than that of control. The flavonoids content was 1.7 times as that of control when the concentration of casein hydmlysate was 0.05 %, and the licochalcone content was 3,2 times as that of control when the concentration of casein hydrolysate was 0.1%. The highest flavonoids content of 149.58μg·g^-1 was obtained with the addition of 2 mL fungal elicitor to the medium, which was 75% increased compared with the control, The JA concentration of 10.0μmol·L^-1 was found to be the most suitable for the flavonoids accumulation with 41% higher than control. Flavonoids content was as 2.7 times as that of the control, and liquiritigenin was as 4 times as that of the control when the concentration of rare-earth element Eu^3＋ was 0.1 mg·L^-1. CONCLUSION The addition of yeast extract, cascin hydrolysate, fungal elicitor, JA and rare-earth element Eu^3＋ to the medium are effective approaches to enhance flavonoids accumulation in G.uralensis calli.

关 键 词：甘草 愈伤组织 酵母提取物 水解酪蛋白 真菌诱导子 茉莉酸 稀土元素 黄酮类化合物 

分 类 号：R931.5[医药卫生—生药学]