羟乙葛根素对大鼠脑缺血再灌注损伤一氧化氮和一氧化氮合酶的影响  

Effects of Hydroxyethylpuerarin on Levels of NO and NOS in Rats with Ischemia-Reperfusion Injury

作　　者：王姿颖[1] 魏欣冰[1] 孙茹[1] 孙霞[1] 张岫美[1] 仲英[2] 左春旭[2] 

机构地区：[1]山东大学医学院药理学研究所,济南250012 [2]山东省医学科学院药物研究所,济南250062

出　　处：《中国药学杂志》2006年第2期112-114,共3页Chinese Pharmaceutical Journal

摘　　要：目的研究异黄酮类化合物羟乙葛根素对大鼠局灶性脑缺血再灌注损伤后脑组织一氧化氮(NO)含量和一氧化氮合酶 (NOS)活性的影响,为羟乙葛根素用于治疗缺血性脑血管病提供实验资料。方法雄性Wistar大鼠60只,随机分为假手术组; 缺血再灌注组;尼莫地平0．4 mg·kg-1·d-1组;羟乙葛根素30,60,120mg·kg-1·d-1组。以大脑中动脉栓线阻断法(MCAO)制备大鼠局灶性脑缺血再灌注损伤模型,以硝酸还原酶法测定脑组织中的NO水平,以化学比色法测定总NOS活性,并测定诱导型一氧化氮合酶(iNOS)活力,观察羟乙葛根素对脑缺血再灌注损伤大鼠脑组织NO水平和NOS活性的影响,并探讨可能的作用机制。结果大鼠脑缺血1 h,再灌注48h后,脑组织中NO水平显著升高,总NOS和iNOS活性显著增加。羟乙葛根素30,60, 120 mg·kg-1·d-1均可显著降低缺血再灌注损伤大鼠脑组织NO含量和总NOS,iNOS活力。结论羟乙葛根素可能通过降低 NO对脑缺血再灌注损伤后的神经毒性作用而发挥保护缺血性脑损伤的作用。OBJECTIVE To investigate the effects of hydroxyethylpuerarin on NO and NOS in rats with focal brain ischemia-reperfusion injury. METHODS Rats were divided into 6 groups randomly： sham-operate group, ischemia-repeffusion group, nimodipine 0.4 mg·kg^-1·d^-1 group and hydroxyethylpuerafin 30,60,120 mg·kg^-1·d^-1 groups. Rats were prepared with focal brain ischemic injury by middle cerebral artery occlusion（MCAO）, then recovered perfusion by pulling out the suture after 1 h. Rats were treated with medcine at 30 min before and 1, 24 and 36 h after operation.48 h after repeffusion, tissue from the forebrain was homogenized, NO and NOS （including total NOS and iNOS） were determined. RESULTS NO and NOS levels were significantly increased in the brain tissue of ischemia-reperfusion group compared with sham-operate group. While being compared with ischemia-reperfusion group, NO and NOS levels were significantly decreased in the three hydroxyethylpuerarin-treated groups. CONCLUSION Hydroxyethylpuemrin can reduce ischemia-reperfusion injury through decreasing the damages of NO.

关 键 词：羟乙葛根素 缺血再灌注 一氧化氮 一氧化氮合酶 

分 类 号：R965[医药卫生—药理学]