季德胜蛇药片对大鼠急性肺损伤的保护作用  

Protective effects of Jidesheng Sheyao tablet on endotoxin-induced acute lung injury in rats

作　　者：陶冬英[1] 陈平圣[1] 刘东风[1] 

机构地区：[1]东南大学基础医学院病理教研室,南京210009

出　　处：《中华急诊医学杂志》2006年第3期223-227,共5页Chinese Journal of Emergency Medicine

基　　金：东南大学国家自然基金预研项目(9223006011);国家自然科学基金资助项目(30470780)

摘　　要：目的研究季德胜蛇药片对细菌脂多糖(Lipopolysaccharide,LPS)诱导大鼠急性肺损伤的保护作用。方法50只雄性SD大鼠随机分为6组:正常对照组、地塞米松阳性对照组、LPS模型组及低、中、高三种不同剂量蛇药组。分别用地塞米松和三种不同剂量的蛇药在LPS诱导ALI模型之前预处理大鼠。检测大鼠的肺系数,光镜观察肺组织损伤程度,酶谱法测肺组织匀浆液和支气管肺泡灌洗液上清中MMP2、MMP9酶活力及免疫组织化学方法检测肺组织MMP2、MMP9蛋白表达情况。结果与模型组比较,阳性对照组及蛇药组肺系数均降低(P<0.05),且高剂量组明显低于阳性对照组(P<0.01)。蛇药组肺组织损伤程度、MMP9酶活力均随剂量的增加而减轻或降低,MMP2酶活力亦有不同程度的下降;高剂量组MMP2、MMP9蛋白表达减弱;结论季德胜蛇药片对LPS诱导大鼠急性肺损伤有一定保护作用,其作用机制可能与抑制MMP2、MMP9表达及活性有关。Objective To investigate the protective role of Jidesheng Sheyao tablet in lipopolysaccharide （LPS） - induced acute lung injury （ALI） in rots. Methods Fifty male SD rots were randomly divided into 6 groups： normal control group, dexamethasone positive group, LPS model group, Sheyao groups of low, middle and high dosages successively. All rats, except normal control group, were administrated with LPS by intravenous injection to induce acute lung injury. The rats in positive group and three different dosage groups were treated by dexamethasone （3 mg·kg^-1, iv）, low dosage （0.8 g·kg^-1）, middle dosage （1.6 g·kg^-1） and high dosage （3.2 g·kg^-1） of Jidesheng Sheyao tablet （oral） respectively before LPS-indueed ALI. Each rat in normal control group received injection of 1ml normal saline. The animals were killed after injection of LPS for 2 hours, and then the lung index was calculated, the histopathology of the lung injury was observed by light microscope, the expression of matrix metalloproteinase-2 （MMP-2）, matrix metalloproteinase-9 （MMP-9） proteins in the lung tissues and their activity in the bronchoalveolar lavage fluid （BALF） and the lung homagenates were detected by immunohistochemisiry and zymography separately. Results Compared with model group, the lung indexes were significantly decreased in the drug groups （P 〈 0.05）; the positive groups were also decreased and was higher than the high dosage group （P 〈 0.01）. The degrees of lung injury including lung swelling, neutrophil cells infiltration and hemorrhage in the drug groups showed fighter in dose-dependent manner than that of model group. The expression of MMP-2 and MMP-9 in lung tissues was obviously inhibited in the high dosage group and their activity in lung homogenate and BALF also presented dose-dependent inhibition （P 〈 0.01） except the activity of MMP-2 in the lung tissue. Condusion Jidosheng Sheyao tablet has protective effects on endotoxin-indueed acute lung injury in rots, and the

关 键 词：季德胜蛇药片 基质金属蛋白酶 急性肺损伤 细菌脂多糖 

分 类 号：R285.5[医药卫生—中药学]