血管紧张素-(1-7)对高血压大鼠内皮功能的影响  

Effects of angiotensin-(1-7) on endothelial dysfunction in rats with coarctation-induced hypertension

作　　者：何建桂[1] 黄艺仪[2] 马虹[1] 廖新学[1] 何小洪[1] 王礼春[1] 

机构地区：[1]中山大学附属第一医院心内科,广州510080 [2]中山大学附属第一医院急诊科,广州510080

出　　处：《中华急诊医学杂志》2006年第3期232-235,共4页Chinese Journal of Emergency Medicine

基　　金：教育部博士点基金资助项目(20020558064);广东省医学科研基金(A2003164)

摘　　要：目的探讨血管紧张素(17)[Ang(17)]对高血压大鼠血管内皮功能的影响。方法采用腹主动脉缩窄术制成高血压大鼠模型,45只SD大鼠分为假手术组、模型对照组、Ang(17)治疗组[25μg/(kg·h),持续给药]。4周后检测动脉血压、血浆NO2-/NO3-、主动脉环对乙酰胆碱(Ach)的舒张反应及主动脉eNOS和iNOS的表达。结果与假手术组比较,模型对照组动脉血压及血浆NO2-/NO3-均明显增高,Ach诱导的主动脉环舒张反应下降,主动脉eNOS表达降低、iNOS表达增加。与模型对照组比较,Ang(17)治疗未改变动脉血压和血浆NO2-/NO3-水平,但改善了主动脉环对Ach的舒张反应,并改善了主动脉eNOS、iNOS的异常表达。结论Ang(17)可改善高血压大鼠的血管内皮功能。Objective To investigate the effects of angiotensin-（1-7） on endothelial dysfunction in rats with coarctation-induced hypertension. Methods Hypertensive rat models were induced by constriction of abdominal aorta. Forty-five male Sprague Dawley rats were randomly divided into sham-operation group, model control group and angiotensin-（1-7） group. They were treated with intravenous infusion of angiotensin-（1-7） （25 mierogram/kg per hour） or saline after operation. The levels of blood pressure and plasma NO2^-/NO3^- were determined at 4 weeks after operation. Acetylcholine （Ach） -induced vasodilation was measured in thoracic aorta. Western blot analysis was performed to valuate the eNOS and iNOS protein expression of aorta. Results The levels of blood pressure and plasma NO2^-/NO3^- in model control group were significantly higher than those of sham-operation group. Compared with sham-operation group, Ach-induced vasodilation was decreased, the expression of eNOS was decreased and the expression of iNOS was increased in model control group. The levels of blood pressure and plasma NO2^-/NO3^- in angiotensin-（1-7） group didn＇t change compared with those in model control group. However, angiotensin-（1-7） improved the vasdilation response of aorta to Ach, and normalized the expression of eNOS and iNOS. Conclusion Angiotensin-（1-7） can improve vascular endothelial dysfunction by promoting eNOS protein expression in rats with coarctation induced hypertension.

关 键 词：血管紧张素-(1-7) 高血压 内皮功能 一氧化氯合酶 

分 类 号：R544.1[医药卫生—心血管疾病]