Non-viral gene delivery carrier of probe ty[pe host molecule--Interactions between DNA and β-cyclodextrin derivative complexes(I)  

作　　者：LIU Tao CHEN Long HOU Sen XUE Yonglai FENG Xizeng 

机构地区：[1]Key Laboratory of Bioactive Materials, Ministry of Education, Collegeof Life Science, Nankai University, Tianjin 300071, China

出　　处：《Chinese Science Bulletin》2006年第5期530-535,共6页

基　　金：supported by the National Natural Science Foundation of China(Grant No.90403140);the Tianjin Natural Science Foundation(Grant No.TJ043801111);the Scientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Education of China(Grant No.B04970).

摘　　要：A host type non-virus gene delivery car- rier, phenanthroline-β-cyclodextrin derivative host molecule, was produced which can be used as mo- lecular probe. Interactions between DZY-1 and DNA were investigated by electrophoresis assay. Hind III enzyme inhibition assay was carried out using DNA condensates induced by host molecules or host- guest molecule complexes to explore their ability to inhibit enzyme digestion. Micro-structure of DNA condensates induced by host molecules and host-guest molecule complexes was observed by scanning electron microscope (SEM). Our work in- dicates the delivery mechanism of DZY-1 used as a gene delivery carrier and also provides a method to design and produce non-virus gene delivery carriers.A host type non-virus gene delivery carrier, phenanthroline-β-cyclodextrin derivative host molecule, was produced which can be used as molecular probe. Interactions between DZY-1 and DNA were investigated by electrophoresis assay. Hind III enzyme inhibition assay was carried out using DNA conclensates induced by host molecules or hostguest molecule complexes to explore their ability to inhibit enzyme digestion. Micro-structure of DNA condensates induced by host molecules and host-guest molecule complexes was observed by scanning electron microscope （SEM）. Our work indicates the delivery mechanism of DZY-1 used as a gene delivery carrier and also provides a method to design and produce non-virus gene delivery carriers.

关 键 词：DNA 邻二氮杂菲-β-环糊精衍生物 非病毒性基因传输载体 琼脂凝胶电泳 扫描电子显微镜 相互作用 

分 类 号：O629.74[理学—有机化学] O636.12[理学—化学]