决明毛状根化学成分研究  

Studies on chemical constituents of hairy root of Cassia obtusifolia

作　　者：杨世海[1] 郭洪祝[2] 果德安[2] 郑俊华[2] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]北京大学药学院,北京100083

出　　处：《中国中药杂志》2006年第3期217-219,共3页China Journal of Chinese Materia Medica

摘　　要：目的:研究决明毛状根的化学成分。方法:用发根农杆菌Agrobacterium rhizogenes9402菌株从决明子叶外植体上诱导产生毛状根,在MS0液体培养基中大量培养获得。并采用色谱技术和波谱手段对毛状根的化学成分进行分离鉴定。结果:从决明毛状根95%乙醇提取物的醋酸乙酯部分分离鉴定了8个化合物,分别是白桦酯酸、大黄酚、大黄素甲醚、豆甾醇、1-羟基-7-甲氧基-3-甲基蒽醌、大黄酚-8-甲醚、大黄酚-1-甲醚和芦荟大黄素。其中芦荟大黄素是首次从决明毛状根中分得。结论:决明毛状根能合成与原植物相似的化学成分。Objective： To study the chemical constituents in hairy roots of Cassia obtusifolia. Method： The hairy roots of C. obtusifolia were induced with Agrobacterium rhizogenes LBA9402 from cotyledons and cultured in MSO liquid medium. The compounds were isolated by silica gel, polyamide and Sephadex LH-20 column chromatography, and the structures were ehucidated by employing chemical and spectral methods Result： Eight compounds were isolated from the ethyl acetate fraction of 95% EtOH extract of the transformed roots of C. obtusifolia. They are betulinic acid, chrysophanol, physcion, stigmasterol, 1-hydroxy-7-methoxy-3-methyl-anthraquinone, 8-O- methylchrysophunol, 1- O-methylchrysophanol and aloe-emodin, and aloe-emodin was isolated from the hairy roots of C. obtusifolia for the fast time. Conclusion The hairy roots of C. obtusifolia have the ability to synthesize the similar chemical constituents as the original plants.

关 键 词：决明 毛状根 化学成分 

分 类 号：R284.1[医药卫生—中药学]