苦豆子总碱对大鼠实验性结肠炎SOD,MDA,NO,MPO表达的影响  

Effects of total base of Sophora alopecuroides on expression of SOD,MDA,NO,MPO in rats with experimental colitis

作　　者：陈建国[1] 邓虹珠[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515

出　　处：《中国中药杂志》2006年第4期323-325,共3页China Journal of Chinese Materia Medica

摘　　要：目的:观察苦豆子总碱对实验性结肠炎大鼠的结肠组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、髓过氧化酶(MPO)表达的影响。方法:将SD大鼠随机分成6组,采用三硝基苯磺酸(TNB)灌肠法,制备实验性结肠炎大鼠模型。第2天开始灌胃,3周后测定结肠黏膜组织中SOD活力、MDA含量、NO含量、MPO活力。评价大鼠的症状和结肠组织学变化。结果:造模组大鼠结肠炎黏膜SOD值显著低于正常组,MDA,NO,MPO值显著高于正常组(P<0.05)。各治疗组均能升高SOD值,降低MDA,NO,MPO值(P<0.05),改善大鼠的症状和结肠组织学损伤(P<0.05)。结论:苦豆子总碱可能通过抑制氧自由基反应,抑制NO生成,抗炎作用来改善或减轻结肠炎症状。Objective： To investigate the effects of total base of Sophora alopeeuroides on expression of SOD, MDA, NO, MPO in rots with experimental colitis （UC）. Method： SD rats were randomized into 6 groups and colitis was induced by administrating 2,4,6-trinitrobenzesulphonic acid（TNB） recatlly in rats. Rats were given drug by stomach after the first day for 3 weeks, and sacrificed at 23^rd day to determine the content of MDA and activities of SOD, NO, MPO in colonic mucosa, and to estimate the symptom and colonic histology. Result： In UC rats, SOD was significantly decreased whereas MDA, NO, MPO were increased when compared with the normal group（ P 〈 0.05）. In all treatment groups, Sophora alopecuroides could increase SOD, decrease MDA, NO, MPO（ P 〈 0.05）, and improve the symptoms and histological damages. Conclusion： The total dose of S. alopecuroides may ameliorates or alleviate the symptoms of UC through inhibiting oxygen free radical reaction, decreasing the generation of NO and exerting anti-inflammatory effects.

关 键 词：苦豆子 结肠炎 超氧化物歧化酶 一氧化氮 髓过氧化酶 

分 类 号：R285.5[医药卫生—中药学]