活动期和稳定期类风湿性关节炎寒热证候患者外周血CD_4^+T淋巴细胞基因表达谱探索  

Comparison on Gene Expression Profile of Periphery CD_4^+ Positive Cells in Active and Stable Stages of Rheumatoid Arthritis

作　　者：肖诚[1,2] 吕诚[1] 赵林华[1] 贾红伟[1] 查青林[3] 闫小萍[2] 王建明[2] 张英泽[2] Youwen Zhou 徐世杰[1] 李梢[5] 吕爱平[1,3] 

机构地区：[1]中国中医科学院基础理论研究所,北京100700 [2]中日友好医院,北京100029 [3]江西中医学院国家工程研究中心,江西南昌330006 [4]University of British Columbia, Vancouver, Canada [5]清华大学生物信息研究所,北京100084

出　　处：《中国中医药信息杂志》2006年第3期14-16,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金委员会重点项目(90209002);国家中医药管理局重点项目(2000-J-Z-02)

摘　　要：目的探讨活动期和稳定期类风湿性关节炎(RA)寒热证候的基因表达差异。方法采集RA寒热证候患者及正常人空腹静脉血,纯化得到CD4+T淋巴细胞,利用基因芯片检测和分析技术,探索活动期和稳定期RA寒热证候患者CD4+T淋巴细胞基因表达差异点。结果RA活动期和稳定期患者有63条基因表达存在显著性差异,主要涉及免疫应答;稳定期RA寒热证候患者之间有48条基因异常表达,只有1条与上述63条基因重复,主要涉及功能代谢;活动期RA寒热证候患者之间有59条基因异常表达,没有与上述稳定期与活动期比较的63条和稳定期寒热证候之间的48条基因重复,主要涉及功能代谢。结论RA患者稳定期和活动期之间的基因表达谱差异与寒热证候之间的基因表达差异有所不同,提示中医证候分类学具有基因表达谱依据。Objective To explore the difference of gene profile between Cold syndrome and Heat syndrome in traditional Chinese medicine in stable and active stage of rheumatoid arthritis （RA）. Methods Peripheral CD4^＋ lymphocytes from RA cases with defined Cold or Heat syndrome in TCM and healthy persons were obtained. Genechip analytical techniques were applied for analysis. Results There are 63 genes found different between active stage and stable stage of RA patients, and they refer to immune responses and signal transduction mainly. 48 genes were found different between Cold syndrome and Heat syndrome in stable stage of RA patients, and only 4 gene was found in the 63 genes for stable and active stage comparison, they are mainly referrirng to functional metabolism. 59 genes were found to be different among Cold syndrome and Heat syndrome in active stage of RA patients, and among them, no gene was same as in the 48 genes for Cold and Heat syndrome comparison in stable stage of RA, nor in the 63 genes for stable and active stage comparison. Conclusion The genes referred with Cold or Heat syndrome in the stable and active stage of RA are different with those genes referring to the difference of gene profile between active stage and active stage of RA, and further suggest that differentiation theory in traditional Chinese medicine have solid foundation in gene expression profile.

关 键 词：类风湿性关节炎 中医证候 基因表达 基因芯片 

分 类 号：R593.22[医药卫生—内科学]