黄芪丹参复方成分对模型孕鼠滋养细胞基质金属蛋白酶9mRNA表达及白细胞介素-10水平的影响  

The Study on the Effects of the Components of Mongolian Milkvetch Root and Red Sage Root Compound on the Expression of Matricial Metal Protease-9 (MMP-9) mRNA on Placental Nourish Cells and the Blood Plasma Level of IL-10 in Model Pregnant Rats

作　　者：王若光[1] 尤昭玲[1] 李春梅[1] 刘小丽[1] 曾润清[1] 秦明春[1] 

机构地区：[1]湖南中医学院中西医结合系,湖南长沙410007

出　　处：《中国中医药信息杂志》2006年第3期41-43,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(30000225);国家中医药管理局基础项目(2000-J-T-17);湖南省教委青年项目(01B014)

摘　　要：目的研究黄芪丹参复方成分提高胎盘血供的分子机制。方法提取黄芪、丹参复方成分,运用一氧化氮合酶(NOS)阻滞剂L-精氨酸甲酯(L-NAME)制作一氧化氮合成阻滞大鼠模型,ELISA法检测妊娠18d大鼠血浆白细胞介素-10(IL-10)水平,核酸原位杂交(FISH)法检测胎盘滋养细胞基质金属蛋白酶9(MMP-9)mRNA表达。结果模型组IL-10含量较空白组低(P<0.01),治疗组IL-10水平高于模型组(P<0.05);模型组MMP-9mRNA表达增加,明显高于空白组和黄芪丹参治疗组(P<0.01),黄芪丹参治疗组与空白组比较差异无显著意义(P>0.05)。结论黄芪丹参复方成分可能通过干预IL-10与MMP-9的互动关系,影响胎盘滋养层细胞的移行与侵袭性,协调胎盘重构与蜕膜重塑过程,从而阻抑胎盘浅表着床,利于母胎循环构建,维持胎盘血液供应。Objective To explore the molecular mechanism of elevating placental blood supply by the component from Mongolian Milkurtch and red sage Root. Method Abstract the effective components from Mongolian Milkurtch and red sage Root, apply L-Arginine methyl ester （L-NAME） to make NO production blocking model, use ELISA to check IL-10 in plasma of 18 d pragant rats, and FISH to check the expression of matricial metal protease-9 （MMP-9） mRNA. Results The plasma level of IL-10 of model group was lower than that of blank group （P〈0.01）, and lower than that of treat group （P〈0.05）, too. While the expression of MMP-9 mRNA of model group was obviously more than that of blank group and treat group （P〈0.01）, but the expression of treat group was not significantly different from that of blank group （P〉0.05）. Conclusion By interring the relationship between IL-10 and MMP-9, the components of Mongolian milkvetch root and red sage root compound may affect the shift and invadation of deciduas, so as to prevent fleet embedding, help building the circulation between mother and embryo, and keep the placental blood supply.

关 键 词：妊娠 一氧化氮 滋养细胞 基质金属蛋白酶类 白细胞介素-10 黄芪 丹参 

分 类 号：R285.5[医药卫生—中药学]