高效液相色谱法测定脑得生胶囊中三七皂苷R_1、人参皂苷Rg_1及人参皂苷Rb_1的含量  

Determination of Notoginsenoside R_1 and Ginsenoside Rg_1, Rb_1 in Naodesheng Capsules by HPLC Gradient Elution Method

作　　者：孙永慧[1] 王晓丽[1] 王建明[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,黑龙江哈尔滨150040

出　　处：《中国中医药信息杂志》2006年第3期50-51,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立高效液相色谱梯度洗脱法测定脑得生胶囊中三七皂苷R1、人参皂苷Rg1及人参皂苷Rb1含量的方法。方法采用色谱柱KromasilC18;以流动相A(乙腈)与流动相B(水)进行梯度洗脱;柱温:25℃。结果三七皂苷R1在0.495～1.782μg(r=0.9999)、人参皂苷Rg1在2.65～9.54μg(r=0.9995)、人参皂苷Rb1在1.85～6.66μg(r=0.9999)范围内呈良好线性关系。加样回收率三七皂苷R1为100.60%(RSD=1.99%),人参皂苷Rg1为100.86%(RSD=2.19%),人参皂苷Rb1为98.83%(RSD=1.95%)。结论本法简便、准确、灵敏度高、重现性好,可用于该制剂的含量测定。Objective To establish a method of HPLC gradient elution method determination of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 in Naodesheng Capsules. Methods Kromasfl C18 column was used with a mobile phase of acetonitrile-water gradient elution, column temperature at 25 ℃. Results Notoginsenoside R1, Ginsenoside Rb1 and Ginsenoside Rg1 showed good linear relationship at the range of 0.495-1.782 μg （r=0.9999）, 2.65-9.54 μg （r=0.9995）, 1.85-6.66 μg （r=0.9999）, respectively. The average recovery. Notoginsenoside R1 was 100.60% （RSD=1.99%）, Ginsenoside Rb1 was 100.86% （RSD=2.19%） and Ginsenoside Rg1 was 98.83% （RSD=1.95%）, respectively. Conclusion The methods is simple, accurate, highly sensitive and reproducible. It can be used for the quantitative determination of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 in Naodesheng Capsules.

关 键 词：脑得生胶囊 三七皂苷R1 人参皂苷RG 人参皂苷RB1 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]