SSR标记对高海拔玉米自交系遗传多样性的研究  

STUDY OF GENETIC DIVERSITY BASED ON SSR MARKERS AMONG MAIZE INBRED LINES ADAPTED TO KARST HIGH ELEVATION MOUNTAINOUS AREAS IN SOUTHWESTERN CHINA

作　　者：彭忠华[1] 赵致[1] 张明生[2] 邱红波 高翔[3] 

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]南京大学生命科学学院,江苏南京210093 [3]贵州省农业科学院,贵州贵阳550025

出　　处：《西南农业大学学报（自然科学版）》2006年第1期17-21,共5页Journal of Southwest Agricultural University

基　　金：贵州省"十五"重大攻关资助项目[2001(1104号)]和(2004NGY023)

摘　　要：利用SSR分子标记技术研究了我国喀斯特高海拔山区主要玉米自交系的遗传多样性，初步进行了杂种优势群划分。37对引物在供试材料中共检测出128个等位基因变异，每对引物检测等位基因2-6个，平均为3．48个，平均多态性信息量为0．506，33个自交系之间的遗传相似系数变化范围为0．476-0．876，平均为0．607。UPGMA聚类分析结果表明，可将我国喀斯特高海拔山区玉米地方自交系划分为6个类群。The technique of SSR markers was used to study the genetic diversity of the main inbred lines of maize in the karst mountainous regions with high elevation. A total of 128 polymorphic bands were detected by 37 primers selected, averaging 3.48 alleles detected per primer. The mean content of polymorphic information was 0.506. Clustering analysis made with UPGMA （ unweighed pair-group method using arithmetic average） classified the maize inbred lines in the karst mountainous regions with high elevation of China into 6 groups.

关 键 词：玉米自交系 SSR标记 喀斯特高海拔山区 遗传多样性 

分 类 号：S513[农业科学—作物学]