球孢链霉菌AM6的液体发酵条件研究  

STUDY ON LIQUID FERMENTATION CONDITIONS FOR STYREPTOMYCES GLOBISPOTUS STRAIN AM 6

作　　者：方敦煌[1] 夏振远[1] 邓建华[1] 

机构地区：[1]云南省烟草科学研究所,云南玉溪653100

出　　处：《西南农业大学学报（自然科学版）》2006年第1期84-86,共3页Journal of Southwest Agricultural University

基　　金：云南省"十五"攻关资助项目(2001NG08);云南省烟草公司重点科研计划资助项目(01A06)

摘　　要：以PD培养液为测定培养基，采用抑菌圈法测定了球孢链霉菌AM6在不同培养时间、pH值、温度、通气量下液体发酵产物时烟草赤星病菌孢子的抑制作用。结果表明，AM6在培养的第96～108h和132-156h时分别达到产孢、产素高峰期；pH9．0,28℃产素最高；通气量少有利于产素。AM 6, a strain of Streptomyces globispotus identified as effective in antagonizing Alternaria alternata in Yunnan in 2001 -2003, was cultured in PD liquid medium with different pH values, culture duration and ventilation volume, and its inhibition effect against A. al- ternata was determined with the inhibition zone method. Spore quantity of AM 6 and the inhibition substances produced by it reached their maximum during 96 ～ 108 h and 132 ～156 h of culture. The optimum pH and temperature for AM 6 growth and inhibition substance production were 9.0 and 28℃, respectively. A lower volume of ventilation was favorable for AM 6 growth and inhibition substance production.

关 键 词：球孢链霉菌AM6 培养时间 PH值 温度 通气量 

分 类 号：S432.43[农业科学—植物病理学]