流动注射化学发光法测定青霉素钠  

Determination of benzylpenicillin sodium by flow injection chemiluminescence method

作　　者：杨一青[1] 马永钧[1] 周敏[1] 张德懿[1] 魏琳琳[1] 陈慧[1] 

机构地区：[1]西北师范大学化学化工学院

出　　处：《药物分析杂志》2006年第2期232-235,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立一种流动注射化学发光快速测定青霉素钠的新方法。方法:在酸性介质中,桑色素与高锰酸钾反应产生强的化学发光,青霉素钠在稀硫酸溶液中降解后,其降解物对该体系的化学发光具有显著的抑制作用,据此建立了利用桑色素-高锰酸钾化学发光体系测定青霉素钠的新方法。结果:化学发光信号的降低值ΔI 与青霉素钠的质量浓度在20.0～100.0mg·L^(-1)范围内呈线性关系,r=0.9993,方法的检出限为3.8mg·L^(-1),绝对发光强度值的 RSD(n=11,C=50.0mg·L^(-1))为0.9%,回收率在98.9%～101.0%之间。结论:用本法对不同产地、不同批号的注射用青霉素钠含量的测定结果,符合中国药典方法的要求。Objective ：To establish a simple and novel method for the determination of benzylpenicillin sodium in pharmaceutical preparations. Method： A novel chemiluminescence （CL） method with flow injection was development for the determination of benzylpenicillin sodium with the morin - potassium permanganate system in sulfuric acid medium. It was based on the significant decreasing of CL intensity of the system by hydrolysate of benzylpenicillin sodium in the acidic medium. Results： The proposed procedure allows quantitation of benzylpenicillin sodium in the range 20. 0 -100. 0 mg· L^-1 with correlation coefficients 0. 9993 （n =6）. The detection limit was 3.8 mg · L^-1 and RSD was 0. 9% （n = 11 ） at 50. 0 mg · L^-1 benzylpenicillin sodium. The benzylpenicillin sodium in injections was measured with fine recoveries ranging from 98.9% to 101, 0%, Conclusion ：This method has been applied to the determination of benzylpenicillin sodium in injections. The results agree well with the standard of Chinese Pharmacopoeia method.

关 键 词：化学发光 青霉素钠 流动注射 

分 类 号：R917[医药卫生—药物分析学]