复方高渗液对低血容量休克大鼠心肌细胞Ca^(2+)浓度及线粒体膜电位的影响  

Effects of compound hyperosmotic solution on calcium concentration and mitochondrial membrane potential in cultured rat myocytes subjected to hemorrhagic shock

作　　者：费奥[1] 滕青山[1] 沈洪[1] 

机构地区：[1]解放军总医院急诊科,北京100853

出　　处：《中国急救医学》2006年第3期190-192,共3页Chinese Journal of Critical Care Medicine

摘　　要：目的探讨复方高渗溶液对低血容量休克大鼠心肌细胞Ca2+浓度及线粒体膜电位变化的影响。方法选用Wistar成年大鼠84只,随机分成高渗复苏组(HS组)和生理盐水复苏组(NS组)两组,制备休克模型后按不同时相(休克前、休克、复苏后51、5、30、60、90 min)处死大鼠6只,取心室肌细胞培养传代,以Fluo-4/AM为游离钙荧光探针JC-1荧光染色,应用流式细胞仪分别检测不同时相心肌细胞Ca2+浓度及线粒体膜电位。结果①HS组在休克,复苏后5、15、30 min各时间点的心肌细胞Ca2+浓度较休克前明显升高,线粒体膜电位较休克前显著降低(P<0.01);在复苏后609、0 min心肌细胞Ca2+浓度和线粒体膜电位与休克时相比差异有非常显著性(P<0.01)。②NS组在休克、复苏后各个时间点同休克前比较心肌细胞Ca2+浓度均明显升高,线粒体膜电位明显下降(P<0.01);复苏后各时间点与休克时比较差异无显著性(P>0.05)。③HS组在复苏后609、0 min心肌细胞Ca2+浓度及线粒体膜电位较NS组差别显著(P<0.01)。结论①低血容量休克可引起大鼠心肌细胞Ca2+浓度升高,线粒体膜电位下降,可导致线粒体功能障碍。②复方高渗液不但能改善低血容量休克大鼠心肌细胞Ca2+浓度,而且能有效稳定其线粒体膜电位;应用生理盐水复苏,对心肌细胞Ca2+浓度及线粒体膜电位作用不显著。Objective To explore the effects of compound hyperosmotic solution on the changes of Ca^2＋ and mitochondrial membrane potential in ctdtured rat myocytes induced by hemorrhagic shook. Methods Eighty - four Wistar rats were randomly divided into two groups ： hyperosmotic solution（HS, n=42） and 0.9% sodium chloride injection（NS, n=42）. At the different timepoint （pre-shook stage, shook, post-resuscitation 5, 15, 30, 60, 90 min）, cardiac myocytes were cultured, fluo 4/AM was loaded as calcium probe, and cells were stained with JC-1. Ca^2＋ and mitochondrial membrane potential were assayed by flow cytomety. Results ①Ca^2＋ of group HS at shook, post-resuscitation 5, 15, 30 min was higher than pre-shock stage, but mitochondrial membrane potential at post-resuscitation 60, 90 min was lower （P〈0.01）. ②Ca^2＋ of group NS at shock and post-resuseitation was higher than pre-shook stage, and mitochondrial membrane potential was lower （P〈0.01）, but there were not significant deviation after resuscitafion as compared with that at shock（ P 〉 0.05 ）. ③Ca^2＋ and mitochondrial membrane potential of group HES at post-resuscitation 60, 90 min were more significantly different than that of group NS （P〈0.01）. Conclusions ①Hemorrhagic shock may lead to the increase of Ca^2＋ and the collapse of mitochondrial membrane potential. So the function of mitochondria will lost. ②Compared with NS, compound hyperesmotie solution can improve Ca^2＋ and stabilize mitochondrial membrane potential.

关 键 词：复方高渗液 休克 心肌 钙浓度 线粒体膜电位 

分 类 号：R605.971[医药卫生—急诊医学]