利用粘红酵母静息细胞高效转化7-ACA成7-ADCA  

Efficient Biotransformation of 7-ACA to 7-ADCA by Resting Cell of Rhodotorula glutinis

作　　者：孙馨蔚[1] 郭长伟[1] 李继安[1] 刘垚[1] 

机构地区：[1]上海医药工业研究院,上海200040

出　　处：《中国医药工业杂志》2006年第3期159-161,共3页Chinese Journal of Pharmaceuticals

摘　　要：采用粘红酵母静息细胞将7-ACA转化为7-ADAC。发酵72h后收集菌体,在底物浓度为6%的pH8.25硼酸盐缓冲液中反应,温度25℃,底物1h内转化完全。分离提取后获得纯度98%的7-ADAC,总收率为88%。7-Aminodeacetylcephalosporanic acid （7-ADAC） was prepared from 7-aminocephalosporanic acid （7- ACA） by biotransformation using resting cell of Rhodotorula glutinis. The cells which had been incubated for 72h were added in borate buffer solution （pH 8.25） containing 6% 7-ACA. The reaction was carried out at 25℃ and completed within lh. The purity of the product reached to 98% with an overall yield of 88%.

关 键 词：粘红酵母 7-氨基头孢烷酸 7-氨基-3-去乙酰基头孢烷酸 生物转化 

分 类 号：R978.11[医药卫生—药品]