缺氧条件下含氮杂环化合物吲哚和吡啶的共代谢研究  

Cometabolic Biodegradation of Nitrogenous Heterocyclic Compounds Indole and Pyridine under Anoxic Conditions

作　　者：李咏梅[1] 李文书[1] 顾国维[1] 

机构地区：[1]同济大学污染控制与资源化研究国家重点实验室,上海200092

出　　处：《环境科学》2006年第2期300-304,共5页Environmental Science

基　　金：国家自然科学基金项目(50108009)

摘　　要：以含氮杂环化合物吲哚和吡啶为研究对象，在传统的缺氧反硝化机理研究基础上，通过向配制废水中加入硝酸盐氮，研究吡啶和吲哚在缺氧条件下的共代谢作用．结果表明，吡啶和吲哚缺氧共代谢的最佳碳氮比为8．4～8．9之间．硝酸还原酶适宜作用的环境条件为：温度28℃，pH值7．0～7．5．吡啶的加入有利于硝酸还原酶活性的提高，吡啶对吲哚的缺氧降解有协同作用．在最佳碳氮比条件下，当吲哚起始浓度为150mg／L，吲哚和吡啶的浓度比例为1～10之间时，吲哚的降解符合零级动力学规律，反应过程中亚硝酸盐氮基本没有积累．当吡啶和吲哚的浓度比小于0．25时，随着吡啶浓度比例的提高，硝酸还原酶活性及吲哚降解速率的增长较快；当吡啶和吲哚的浓度比大于0．25时，硝酸还原酶活性及吲哚降解速率的增长变得比较缓慢．Based on the traditional theory of denitrification under anoxic conditions, and with the addition of nitrate, experiments were conducted to investigate the cometabolic biodegradation of nitrogenous heterocyclic compounds-indole and pyridine. Results show that the optimum ratio of COD to nitrate （C/N） is 8.4-8.9. Under the conditions with temperature of 28℃ and pH of 7.0-7.5, the nitrate reductase activity （NRA） is in the best state. Addition of pyridine can promote NRA and the degradation of indole. When the initial concentration of indole is 150mg/L, the concentration ratio of indole to pyridine is 1- 10, and under the optimum C/N conditions, the degradation of indole meet with zero-order kinetics. There was no accumulation of nitrite during the reaction. When the concentration ratio of pyridine to indoh is less than 0.25, with the increase of pyridine concentration, there is a faster augment rate for NRA and the degradation of indole than the situation when the concentration ratio of pyridine to indole is more than 0.25.

关 键 词：吲哚 吡啶 共代谢 缺氧反硝化 硝酸还原酶活性 

分 类 号：X703.1[环境科学与工程—环境工程]