异养硝化细菌的分离及其硝化特性实验研究  

Isolation and Characterization of Heterotrophic Nitrifying Bacteria

作　　者：林燕[1] 孔海南[1] 何义亮[1] 严立[1] 李春杰[1] 

机构地区：[1]上海交通大学环境科学与工程学院,上海200240

出　　处：《环境科学》2006年第2期324-328,共5页Environmental Science

基　　金：国家自然科学基金项目(20176027)

摘　　要：采用将传统微生物学方法与现代分子生物学手段相结合的新型异养硝化细菌的分离筛选方法,从具有80.1%的SND效果的MBR系统中分离筛选出2株异养硝化细菌,通过生理生化实验、16S rDNA的序列分析,认定为属新报道的异养型硝化细菌.通过批式试验考察了分离纯化得到的异养硝化细菌的硝化性能.结果表明,Bacillussp.LY及Brevibacillussp.LY具有异养微生物的性质,在充分利用有机碳进行有氧呼吸的同时还具有较强的硝化及脱氮能力:24d好氧培养后,COD的去除率分别为71.7%及52.6%;氨氮的转化率分别为78.2%及51.2%;总氮的转化率分别为69.2%及35.6%.Method of isolating the heterotrophic nitrifiers and the characterization of the heterotrophic nitrification were studied. Two heterotrophie nitrifiers were newly isolated from a membrane bioreactor （MBR） in which the TN removal efficiency was 80.1%. The batch test results indicate that Bacillus sp. LY and Brevibacillus sp. LY could utilize the organic carbon as the source of assimilation when they grew on glucose and ammonium chloride medium companying the formation of oxidized-nitrogen. After 24 days incubation, the removal efficiencies of the COD by Bacillus sp. LY and Brevibacillus sp. LY were 71.7% and 52.6%, respectively. The removal efficiencies of ammonium nitrogen by the two isolates were 78.2% and 51.2% and the TN removal efficiencies by the two isolates were 61.2% and 35.6 %, respectively.

关 键 词：分离 BACILLUS SP LY Brevibacillus sp.LY 异养硝化 硝化特性 

分 类 号：X703.1[环境科学与工程—环境工程]