氯菊酯农药间接酶联免疫吸附测定法的建立  

Indirect ELISA for the Pyrethroids-Permethrin Residue Detection

作　　者：刘廷凤[1] 刘亚子[1] 孙成[1] 

机构地区：[1]南京大学环境学院污染控制与资源化国家重点实验室,南京210093

出　　处：《环境科学》2006年第2期347-350,共4页Environmental Science

基　　金：国家自然科学基金项目(29877010);江苏省科学基金项目(BK2002088)

摘　　要：为测定环境样品中氯菊酯农药残留，用活泼酯法将氯菊酯半抗原（Py）与卵白蛋白（OVA）偶联，制备合成抗原Py-OVA作包被原，建立间接竞争酶联免疫吸附测定法．方阵滴定确定了抗血清最佳稀释度（1：2500），包被抗原的最适工作浓度0．45μg／mL，并建立了标准工作曲线．工作曲线表明在10-800μg／L浓度范围内呈良好的线性关系，回收率〉97％．In order to detect the permethrin （Py） residue in environment samples, Ovalbumin（OVA）was used as protein carrier to couple with semi-antigen permethrin by active ester method. Indirect competitive ELISA （icELISA） was established. The most appropriate titration of coating antigen was 0.45μg/mL and optimal dilution was 1/2 500 correspondingly. The standard curve of icELISA was also established and the curve indicated that the lowest detection limit was 0. 1μg/mL, the curve had a favorable linear relation with the concentration range of 10-800μg/mL, recoveries of permethrin （ 〉 97 % ） were satisfactory.

关 键 词：ELISA 拟除虫菊酯 农药 残留分析 

分 类 号：X839.2[环境科学与工程—环境工程]