Confocal epifluorescence detection for microspheres delivered on disposable microfluidic chip  

作　　者：胡宏华 侯西云 杨国光 

机构地区：[1]State Key Laboratory of Modern Optical Instrumentation,Zhejiang University, Hangzhou 310027 [2]State Key Laboratory of Modern Optical Instrumentation,Zhejiang University,Hangzhou 310027

出　　处：《Chinese Optics Letters》2006年第3期177-180,共4页中国光学快报（英文版）

基　　金：This work was funded by the Microsystem Center of Zhejiang University.

摘　　要：The laser induced fluorescence （LIF） detection system for 5-μm microspheres delivered on microfluidic chip is presented employing eonfocal optical scheme. The parameters of the optical system are specifically optimized for single microsphere detection. With the excitation laser spot size of 4.6 μm and optical sectioning power of 27 μm, the lowest concentration detection limit is 0.45 nmol/L, corresponding to only 122 molecules in probe volume. The microsphere detection is carried on successfully with the maximum signal-to-noise ratio （SNR） of 55.7, which provides good detection sensitivity.The laser induced fluorescence （LIF） detection system for 5-μm microspheres delivered on microfluidic chip is presented employing eonfocal optical scheme. The parameters of the optical system are specifically optimized for single microsphere detection. With the excitation laser spot size of 4.6 μm and optical sectioning power of 27 μm, the lowest concentration detection limit is 0.45 nmol/L, corresponding to only 122 molecules in probe volume. The microsphere detection is carried on successfully with the maximum signal-to-noise ratio （SNR） of 55.7, which provides good detection sensitivity.

关 键 词：MOLECULES Optical systems Signal to noise ratio 

分 类 号：TN492[电子电信—微电子学与固体电子学]