猪传染性胸膜肺炎放线杆菌毒素I基因的克隆、序列分析及表达  

Cloning,sequencing and expression of the apxⅠgene of Actinobacillus pleuropneumonia

作　　者：王芳[1] 何孔旺[1] 邱索平[1] 彭小华[1] 倪艳秀[1] 张雪寒[1] 郭容利[1] 俞正玉[1] 

机构地区：[1]江苏省农业科学院兽医研究所农业部畜禽疫病诊断重点开放实验室,江苏南京210014

出　　处：《中国预防兽医学报》2006年第2期121-123,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：江苏省创新人才基金(BK2003424)

摘　　要：参照猪传染性胸膜肺炎放线杆菌血清型Ⅰ菌株序列设计了一对特异性引物,用PCR方法扩增毒素Ⅰ基因,得到约920bp的片段,与参考序列的核苷酸同源性达99.3%,定向克隆到pET32a(+)中,转化BL21(DE3)To amplify apx Ⅰ gene by PCR pair of primers were designed.The amplified DN from serotype 1 strain of Actinobacillus pleuropneumonia （App）,a A fragment 920 bp was cloned into expression vector pET-32a and sequenced. The result of sequencing showed that the homology was 99.3 % compared with the reference sequence. The recombinant plasmid was transformed in Escherichia coil BL21 （DE3）. After induced,the recombinant protein was expressed in BL21 at high level,and the expressed protein was positive by Western blot.

关 键 词：胸膜肺炎放线杆菌 毒素Ⅰ 表达 

分 类 号：S852.61[农业科学—基础兽医学] Q785[农业科学—兽医学]