乌梅丸对溃疡性结肠炎大鼠组织细胞因子的干预效应  

Interventional effect of wumei wan on cytokines in the colon tissues of rats with ulcerative colitis

作　　者：范恒[1] 邱明义[2] 梅家俊[2] 沈关心[3] 刘松林[2] 寿折星[1] 吕建芳[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中西医结合科,湖北省武汉市430022 [2]湖北中医学院中医系,湖北省武汉市430061 [3]华中科技大学同济医学院免疫教研室,湖北省武汉市430030

出　　处：《中国临床康复》2006年第7期87-89,共3页Chinese Journal of Clinical Rehabilitation

基　　金：湖北省教委资助项目(99Z014)~~

摘　　要：目的:观察中草药乌梅丸对溃疡性结肠炎大鼠组织促炎细胞因子肿瘤坏死因子α、白细胞介素6,8和抗炎细胞因子白细胞介素10的影响,并比较乌梅丸与柳氮磺胺吡啶治疗效果。方法:实验于2002-10/2003-03在湖北中医学院中心实验室完成。①选用健康SD大鼠56只,雌雄各半。按雌雄随机分4组:正常组、乌梅丸组、柳氮磺胺吡啶组、模型组,每组14只(雌雄各半)。应用2,4-二硝基氯苯免疫加醋酸局部灌肠法建立溃疡性结肠炎大鼠模型,除正常组外,其余组均造模。②乌梅丸组:给予配制的乌梅丸液(中草药乌梅丸为本院自制,成分:乌梅16g,细辛6g,干姜10g,黄连16g,当归4g,附子6g,蜀椒4g,桂枝6g,生晒参6g,黄檗6g。按传统方法配制成含生药浓度分别为515g/L的水煎剂)3mL灌胃,1次/d;柳氮磺胺吡啶组:给予柳氮磺胺吡啶混悬液3mL在造模后灌胃,1次/d;模型组和正常组:均以蒸馏水3mL灌胃,1次/d。各组均给药15d。③然后于第16天,采用酶联免疫吸附法检测结肠组织白细胞介素6,8,10,肿瘤坏死因子α含量。④计量结果差异比较采用t检验、方差分析(q检验)。结果:由于每组大鼠在灌胃前随机抽取2只进行病理检查以及部分标本采集不合格,正常组、模型组、柳氮磺胺吡啶组、乌梅丸组分别脱失2,4,5,3只,最终进入结果分析以上各组大鼠分别为12,10,9,11只。①大鼠结肠组织白细胞介素6,8,肿瘤坏死因子α含量:模型组均明显高于正常组(P<0.01),柳氮磺胺吡啶组和乌梅丸组明显低于模型组(P<0.01),乌梅丸组明显低于柳氮磺胺吡啶组(P<0.01)。②大鼠结肠组织白细胞介素10含量:模型组明显低于正常组(P<0.01),柳氮磺胺吡啶组和乌梅丸组明显高于模型组(P<0.01),乌梅丸组明显高于柳氮磺胺吡啶组(P<0.05)。结论:乌梅丸有上调抗炎细胞因子白细胞介素10,下调促炎细胞因子肿瘤坏死因子α、白细胞介素6,8的作用,使溃疡性结肠炎�AIM： To observe the effect of a Chinese herb wumei wan （WMW） on proinflammtory cytokines tumor necrosis factor alpha （FNF-α, interleukin- 6 （IL-6）, IL-8, and imnmnasuppressive cytokine IL-10 of rats with ulcerative colitis （UC）, and compare the therapeutic effect of WMW with solfasalazine （SASP）. METHODS： The experiment was completed in the Central Laboratory of Hubei College of Traditional Chinese Medicine from October 2002 to March 2003. ① Totally 56 healthy SD rats of half in each gender were randomly divided＇into 4 groups： normal group, WMW group, SASP group and model group with 14 in each group. Except rats in normal group, rats in other three groups were established UC models with local enema of 2,4- chlorodinitrobenzene immune and acetic acid. ② WMW group： Rats in WMW group were perfused with 3 mL WMW fluid, once a day （Herbs quantity in WMW were as follows： 16 g dark plum, 6 g asarum herb, 10 g dried ginger, 16 g Chinese goldthread, 4 g Chinese angelica root, 6 g aconite root, 4 g pricklyash peel, 6 g cassia twig, 6 g sun-dried ginseng, 6 g bark of cork tree. Decoction in which raw drug contents were 515 g/L, were prepared based on WMW with the traditional method）. SASP group： Rats in SASP group were perfused with 3 mL SASP once a day after modeling. Model group and normal group： Rats were perfused with 3 mL distilled water once a day for 15 days. ③ On the 16z day, contents of cytokines IL-6, IL-8, IL-10 and TNF-α in colon tissue were measured with enzyme-linked immuno-sorbent assay. ④ All data were analyzed with t test and variance analysis （q-test）. RESULTS： Because 2 rats in every group were killed for pathologic testing before lavage and collection of the part specimenswas not right. Totally 2, 4, 5 and 3 rats were missed respectively in normal group, model group, SASP group and WMW group. Finally, 12, 10, 9 and 11 rats were involved in the analysis of results. ① Contents of IL-6, IL-8 and FNF-α in colon tissue： Contents in model group were hi

关 键 词：中药学 结肠炎 溃疡性/中药疗法 肿瘤坏死因子 乌梅丸/治疗应用 乌梅丸/药理学 肿瘤坏死因子/代谢 白细胞介索6/代谢 白细 胞介素8/代谢 白细胞介紊10/代谢 

分 类 号：R656.9[医药卫生—外科学]