高脂饲料和高果糖餐分别诱导胰岛素抵抗大鼠模型的胰岛素敏感性  

Sensitivity to insulin in rat models of insulin resistance induced by high fat feed and high laevulose forage

作　　者：程海波[1] 司晓晨[1] 尚文斌[1] 卞慧敏[2] 

机构地区：[1]南京中医药大学第一临床医学院,江苏省南京市210029 [2]南京中医药大学植物药研究与新药开发中心,江苏省南京市210029

出　　处：《中国临床康复》2006年第7期121-123,共3页Chinese Journal of Clinical Rehabilitation

基　　金：江苏省普通高校自然科学研究计划资助项目(99KJB360001);江苏省中医药管理局科学技术研究资助项目(9924)~~

摘　　要：目的:建立高脂饲料和高果糖餐诱导的胰岛素抵抗大鼠模型,分别评定两种动物模型的胰岛素敏感性。方法:实验于2004-03/06在南京中医药大学第一临床医学院中医内科急难症研究所完成,选用Wistar雄性大鼠72只,适应性饲养1周后,随机分为3组,即空白组、果糖模型组和高脂模型组,每组24只。空白组继续以普通标准大鼠饲料喂养,饮用自来水;果糖模型组以100g/L的果糖水取代自来水喂养;高脂模型组以高脂饲料取代普通标准大鼠饲料喂养,连续6周。每周测量各组大鼠的体质量、血压。6周结束时,分别测定各组大鼠葡萄糖耐量、血清游离脂肪酸、三酰甘油、血浆胰岛素,同时以正常血糖-高血浆胰岛素钳夹技术,结合输注3H-2DG测定股四头肌葡萄糖摄取率评定大鼠的胰岛素敏感性。胰岛素敏感性指数=1n1/(空腹血糖×空腹胰岛素)。结果:纳入72只大鼠全部进入结果分析,无脱失。①各组大鼠体质量、血压比较:与空白组大鼠比较,高脂模型组体质量显著增加(t=2.819～11.430,P<0.01),果糖模型组血压显著升高(t=4.743～14.510,P<0.01)。②各组大鼠糖耐量比较:果糖模型组和高脂模型组大鼠的葡萄糖耐量显著低于空白组(t=2.833,4.030,P<0.05,P<0.01)。③各组大鼠血清三酰甘油、游离脂肪酸、血浆胰岛素含量比较:果糖模型组和高脂模型组大鼠显著高于空白组(t=3.059～10.391,P<0.01)。④各组大鼠胰岛素敏感性指数、葡萄糖输注率、葡萄糖摄取率比较:果糖模型组和高脂模型组大鼠显著低于空白组(t=2.212～58.052,P<0.01)。结论:高脂饲料和高果糖餐喂饲均可以诱导胰岛素抵抗大鼠模型。AIM： To set up the rat models of insulin resistance induced by high fat feed and high laevulose forage respectively and evaluate the insulin sensitivity of the two kinds of models. METHODS： The experiment was conducted at the Institute of Acute and Complex Disease, Department of Chinese Internal Medicine, the First Clinical Medical College of Nanjing University of Traditional Chinese Medicine between March and June 2004. After one-week adaptive breeding, 72 male Wistar rats were divided into three groups randomly： control group, laevulose group and fat group, with 24 in each group. The rats in the control group were fed with normal rat forage and tap water, while 100 g/L laevulose water for the laevulose group and high fat forage for the fat group, continuously for six weeks. Body mass （BM） and blood pressure （BP） were detected each week. After six weeks, the glucose tolerance （GT）, free fatty acids （FFA）, triacylglycerol （TG） andinsulin （INS） were surveyed. The glucose uptake rate （GUR） of quadrieeps femoris was determined by a combination of euglyeemic insulin clamp technique （EICT） and 3H-2DG infusions to evaluate the insulin sensitivity of rats. The insulin sensitivity index （ISI）=ln 1/（fasting blood glucose χ fasting insulin）. RF, SULTS： Totally 72 rats were involved in the result analysis without drop. ①Comparison of BM and BP： Compared with the control group, the BM of rats increased obviously in the fat group （t=2.819-11.430,P 〈 0.01）, and BP markedly increased in the laevulose group （t=4.743-14.510, P 〈 0.01）.② GT comparison： GT were significantly lower in the laevulose group and the fat group than in the model group （t=2.833,4.030,P 〈 0.05,P 〈 0.01）. ③Compafisons of TG, FFA and INS levels： The levels of TG, FFA and INS were higher obviously in the laevulose group and the fat group than in the model group （t=3.059-10.391 ,P 〈 0.01）.④Comparisons of the ISI, glucose infusion rate （GIR） and GUR： The ISI,GIR and GU

关 键 词：胰岛素抗药性 疾病模型 动物 膳食 果糖 

分 类 号：R587.1[医药卫生—内分泌]