bFGF/BMP/AACB复合物修复犬股骨头缺损坏死模型的实验研究  

Defects and necrosis of canine femoral head repaired with tissue engineering bone

作　　者：龚跃昆[1] 彭吾训[1] 李彪[1] 刘劲松[1] 李世和[1] 

机构地区：[1]昆明医学院第一附属医院骨科,650032

出　　处：《中华创伤骨科杂志》2006年第3期248-251,共4页Chinese Journal of Orthopaedic Trauma

基　　金：云南省教育厅科学研究基金资助项目(02ZY251;03Z496C;04Z855C)

摘　　要：目的制备bFGF/BMP/AACB复合物,通过动物实验研究其对股骨头缺损坏死模型的修复作用及相关问题,探讨临床应用的可能性。方法制备异体脱抗原松质骨(AACB),并将其与碱性成纤维细胞生长因子(bFGF)及骨形态发生蛋白(BMP)复合制成bFGF/BMP/AACB复合物,植入通过液氮冷冻建立的犬股骨头缺损坏死模型,术后不同时间处死动物。通过大体解剖、X线片、组织学染色、图像分析、血管免疫组化染色、X线能谱分析、生物力学测定等手段观察缺损坏死模型的修复情况。结果术后各组动物均无毒性反应和炎症反应。A组(空白缺损组)术后12周缺损仍未愈合;B组(AACB组)虽然优于A组,但成骨作用不及C组(BMP/AACB组);而D组(bFGF/BMP/AACB组)成骨和成血管作用优于A、B、C组。早期(3、6周)甚至优于E组(自体骨组),后期(12周)的骨修复及骨改建效果与E组相当。结论bFGF/BMP/AACB复合物具有明显促进犬股骨头缺损坏死模型再血管化及新骨形成的作用,可望成为新型的股骨头缺血性坏死(ANFH)修复材料。Objective To evaluate a new type of bone graft material made by combining bFGF, BMP with AACB in the clinic repair of ANFH（avascular necrosis of the femoral head） in animal experiments. Methods The combined material of bFGF/BMP/AACB was made and then this composite was transplanted to the models of canine femoral head defects and necrosis established by liquid nitrogen freezing, The specimens were harvested respectively at the end of three, six and 12 weeks after operation. A series of examinations were carried out to evaluate the repair results, including gross observation, radiography, histology, image pattern analysis, blood vessel immuhistochemical staining, EDAX （energy dispersion analysis X-ray）, and mechanical measurement. Results All the animals had no toxic or inflammatory reactions. Little new bone formed in group A （control group） by the end of 12 weeks. Group B （AACB group） was better than group A, and group C （BMP/AACB group） better than group B in osteogenesis. All the results showed that group D （bFGF/BMP/AACB group） was much superior to groups A, B and C in angiogenesis and osteogenesis at all intervals, and even better than group E （bone autograft group） by the end 6f three weeks and six weeks. By the end of 12 weeks, bone repairing and remodeling effects in group D were as good as in group E. Conclusions Since the bFGF/BMP/AACB compsite has great potential for enhacing revascularization and osteogenesis in repairing the defect and necrosis model of canine femoral head, it can be used as a substitute bone graft material for clinical patients with ANFH.

关 键 词：股骨头缺血性坏死 碱性成纤维细胞生长因子 骨形态发生蛋白 异体脱抗原松质骨 

分 类 号：R681.8[医药卫生—骨科学]