兔颈部硬脊膜表面及椎板旁神经分布的实验研究  

Experimental study of nerve fibers' distribution and innervation on the surface of dura mater of spinal cord and lamina of vertebra in rabbits

作　　者：左金良[1] 韩建龙[1] 韩庆森[1] 马英文[1] 秦川[1] 

机构地区：[1]济南市脊柱外科研究所济南市第四人民医院骨科,250031

出　　处：《中国脊柱脊髓杂志》2006年第1期62-64,F0003,共4页Chinese Journal of Spine and Spinal Cord

摘　　要：目的:观测兔颈部硬脊膜表面及椎板旁的神经分布规律,为颈性眩晕发病机理的研究提供实验依据。方法:20只新西兰兔,分为硬脊膜实验组及对照组、椎板旁实验侧及对照侧,分别于实验组(侧)(C6硬脊膜或C5椎板表面)喷涂辣根过氧化物酶(HRP)溶液、而对照组(侧)喷涂生理盐水,48h后切取双侧颈上神经节、颈下神经节(或星状神经节)及双侧脊神经节,按TMB法进行成色反应,光镜观察HRP标记细胞形态及其分布情况。利用辣根过氧化物酶逆行示踪技术,了解硬脊膜表面及椎板旁的神经分布规律。结果:硬脊膜实验组双侧C5～C7脊神经节内出现HRP标记细胞,椎板旁实验侧同侧C4～C6脊神经节内出现HRP标记细胞,交感神经节及对照组(侧)脊神经节内未出现标记细胞。结论:颈部硬脊膜表面的神经主要来自双侧脊神经节,相邻节段间有重叠分布;椎板旁的神经主要来自同侧脊神经节,且相邻节段间有重叠。Objective：To define the protocol of nerve innervation on the surface of dura matter of spinal cord and lamina of vertebra in rabbits and provide experimental evidence for studying the mechanism of cervical vertigo.Method：20 New Zealand rabbits were divided into 4 groups（2 experimental groups and 2 control groups） randomly.Horseradish peroxidase（HRP） solution was injected onto the surface of C6 dura matter or the surface of C5 vertebra lamina.The superior cervical ganglia ,inferior cervical ganglia or stellate ganglia and spinal ganglias of both sides were removed,processed by frozen section and TMB method.The HRP labeled cells in sections were observed with optical microscope,which were used to define the innervation of nerve fiber on the surface of dura matter of spinal cord and lamina of vertebra by using the method of retrograde axonal transport of HRP.Result：The HRP labeled cells were found in C5-C7 spinal ganglias of both sides in dura matter groups and in C4-C6 spinal ganglias of the experimental side in vertebra lamina groups.But the labeled cell was not found in the sympathetic ganglias and spinal ganglias of control groups.Conclusion：The nerve fibers on the surface of the cervical dura matter of spinal cord originate come from the spinal ganglias of both sides,and the nerve fibers on the surface of the vertebra lamina from the cervical ganglias of the same side,there is overlap for the fibers of adjacent spinal ganglias on the surface of dura matter or vertebra lamina.

关 键 词：辣根过氧化物酶 神经分布 硬脊膜 颈性眩晕 

分 类 号：R954.52[医药卫生—药学] R319