变应性鼻炎特异性IgE与IL-13单核苷酸多态性的相关性研究  

A study of the correlation between serum-specific IgE and IL-13 single nucleotide polymorphism among the patients with allergic rhinitis

作　　者：邢志敏[1] 王旻[1] 于德林[1] 闫征[1] 刘晓玲[1] 余力生[1] 

机构地区：[1]北京大学人民医院耳鼻咽喉科,北京100044

出　　处：《中国耳鼻咽喉头颈外科》2006年第1期43-46,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：北京大学疾病相关基因研究中心985课题资助项目(2001-5)

摘　　要：目的探讨对屋尘螨和蒿属花粉过敏的变应性鼻炎患者特异性IgE与IL-13单核苷酸多态性(singlenucleotidepolymorphism,SNP)的相关性。方法对69例屋尘螨过敏为主和87例对蒿属花粉过敏为主的变应性鼻炎患者进行特异性IgE测定,基因型的鉴定采用限制性片段长度多态性(restrictionfragmentlengthpolymorphism,RFLP)的方法,检测IL-13基因启动子的-1112(C/T)位点和编码区的Arg130Gln位点的两个SNP位点,并探讨特异性IgE与这两个位点的相关性。结果-1112(C/T)同屋尘螨的特异性IgE增高有相关性(P=0.041),未发现Arg130Gln位点同血清中的屋尘螨的特异性IgE增高有相关性;两位点同蒿属花粉的特异IgE之间均没有相关性。结论-1112(C/T)同屋尘螨的特异性IgE增高有相关性,提示该位点可能参与螨特异性IgE的调控。OBJECTIVE To study the correlation between the serum-specific IgE and IL-13 single nucleotide polymorphism （SNP） among patients with allergic rhinitis who were allergic to Dermataphagoides pteronyssinus（Derpl） or Artemisia Pollen. METHODS Sixty nine patients who were allergic to Derpl and 87 patients who were allergic to pollen were examined for detecting serum-specific IgE by a fluorescent enzyme immunoassay. The IL-13 SNP of the coding sequence （Arg130GIn） and the promoter region -1112 （C/T） was measured by polymerase chain reaction/restriction fragment length polymorphism （PCR-RFLP） techniques. RESULTS Among patients who were allergic to Derpl, there was a significant correlation between the IL-13 promoter -1112 （C/T） SNP ,but not of Arg130GIn, and serum-specific IgE. Among patients who were allergic to pollen, there were no significant asso.ciation both of the IL-13 promoter -1112 （C/T） and Arg130GIn SNPs with serum-specific IgE. CONCLUSION The results showed a significant association of the IL-13 promoter-1112 （C/T） SNP with serum-specific IgE in patients who allergic to Derpl. That suggested a possible involvement of 1L-13 promoter -1112 （C/T） SNP in the regulation of serum-specific IgE response to Derpl allergens.

关 键 词：鼻炎 变应性 常年性 白细胞介素13 单核苷多态现象 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]