大鼠原位肝移植胆道外引流及胆汁采集方法的改进与评估  

Modification of external bile drainage and bile collection method in rat orthotopic liver transplantation model

作　　者：陈耿[1] 张玉君[1] 杨程[1] 郑树国[1] 李昆[1] 李晓武[1] 董家鸿[1] 

机构地区：[1]第三军医大学西南医院全军肝胆外科研究所,中国人民解放军西南肝胆外科医院,重庆400038

出　　处：《消化外科》2006年第2期129-132,共4页Journal of Digestive Surgery

基　　金：国家自然科学基金资助(No.30571834)

摘　　要：目的改进传统的大鼠肝移植胆道外引流及胆汁采集方法,为肝移植术后胆道问题的研究提供更加符合生理的动物模型。方法47例大鼠原位肝移植,其中27例采用改进术式(实验组):在传统肝移植胆道外引流的基础上,加行空肠造瘘,并在体外建立胆汁回流通路,术后通过自制的限制性大鼠笼固定大鼠以便采集胆汁;其余20例仅单纯行肝移植术(对照组)。结果实验组手术成功率96.3%,术后1、2周动物存活率分别为85.19%、81.48%,与对照组接近。两组大鼠术后胆汁γ-GT、ALP、总胆汁酸及主要胆盐水平差异均无统计学意义。结论通过加行空肠造瘘及建立体外胆汁回流通路,能够在不显著加重胆管损伤的情况下有效维持肠肝循环。该模型比较符合生理,易于标准化,为研究肝移植术后免疫排斥和胆道问题创造了良好的条件。Objective To modify the traditional methods for external bile drainage and the bile collection in rat orthotopic liver transplantation （OLT） model. Methods Of 47 rat OLTs, 20 were performed with conventional two-cuff technique （control group）, 27 were combined with external bile drainage and enterostomy （experimental group）. In the experimental group, a self-made connector was used to rebuid in vitro bile flow bypass, and all rats were placed in self-made restraining cages for bile collection. Results The successful rate of OLT in the experimental group was 96. 3%, and the posttransplant 1-week and 2-week survival rates was 85.19% and 81.48% respectively, which was comparable to the control group. There were no significant difference in bile γ-GT, ALP, total bile acid and major bile salts levels between these the two groups. Conclusions The modified technique of rebuiding in vitro bile flow bypass can maintain the enterohepatic circulation effectively without the aggravation of bile duct injury. This modified rat OLT model is physiological and easy to standardize.

关 键 词：肝移植 胆道外引流 胆汁采集 

分 类 号：R657.3[医药卫生—外科学]