SPE-HPLC用于蔬菜中甲基对硫磷和对硫磷同时测定  

Simultaneous Determination of Methylated Parathionin and Parathionin Vegetables by SPE-HPLC

作　　者：陶宏亮[1] 关燕萍[1] 苏晓峰[1] 柳训才[1] 韦明元[1] 李胜清[1] 陈浩[1] 

机构地区：[1]华中农业大学理学院,武汉430070

出　　处：《华中农业大学学报》2006年第1期46-49,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(40271064);华中农业大学大学生科技创新项目(2004A076)资助

摘　　要：采用乙腈提取，ENVI-18固相萃取，丙酮洗脱，C18高效液相色谱分离柱，甲醇-水（体积比85：15）流动相，紫外检测波长275nm，建立了同时测定蔬菜中甲基对硫磷和对硫磷残留量的固相萃取-高效液相色谱法。结果表明：测定甲基对硫磷和对硫磷农药残留量的线性范围为0．05～5．00mg／L，相关系数0.9998～0．9999，检出限0．025～0．031mg／L，加标回收率81．60-93．60％，相对标准偏差5．3％～2．9％。Residues of two organophosphorus pesticides, nearly parathion-methyl and parathion, in vegetables were determined by high performance liquid chromatography （HPLC） with an off-line solid phase extraction （SPE） ENVI-18 SPE columns. Acetone was used to elute the pesticides from the SPE columns, Chromatographic separation was performed in ZORBAX Eclipse XDB-C18 4. 6mm × 150 mm, 5 um with an eluent consisting of methanol - water with the volume ratio of 85 ： 15 . The eluent was detected with UV detector at 275 nm. Results obtained with the recoveries greater than 80%, the entire analytic time less than 2 h, and the detection limit between 0. 025 mg/L and 0. 031 mg/L were ideal. The method can be used to determine the parathion-methyl and parathion in vegetables quickly and accurately.

关 键 词：固相萃取 高效液相色谱法 甲基对硫磷 对硫磷 蔬菜 测定 

分 类 号：S132[农业科学—农业基础科学] S601